364 papers
This study reveals that DNA-PK-mediated phosphorylation of topoisomerase IIβ (TOP2B) at T1403 regulates DNA topology to repress hypoxia-inducible gene expression under normoxia, while TOP2B dissociation and DNA-PK/HIF1 recruitment under hypoxia activate these genes.
This paper demonstrates that both template matching and deep neural network-based particle picking methods in cryo-EM and cryo-ET can generate persistent molecular structures from pure noise due to confirmation bias, thereby highlighting a critical vulnerability in current workflows and proposing mitigation strategies to ensure data integrity.
This study reveals that diverse bacterial STAND NTPase families function as a comprehensive immune system by recognizing a wide array of conserved phage structural and replicative proteins, often repurposing host factors like EF-Tu to assemble defense complexes.
This study reveals that nucleosomes actively stimulate DNA polymerase β activity in linker DNA and coordinate a multi-layered regulatory system involving PARP1/2 and histone H1 to modulate base excision repair pathway choice and overcome chromatin-mediated inhibition.
This study reveals that the long noncoding RNA FENDRR fine-tunes FOXF1 protein levels through a negative feedback loop—where FOXF1 activates FENDRR transcription and FENDRR subsequently suppresses FOXF1 protein stability—to precisely regulate human embryonic lung fibroblast-to-myofibroblast transition, providing a mechanistic explanation for the dosage-sensitive lung defects observed in ACDMPV.
This study employs a multi-objective engineering strategy to enhance the thermostability and cofactor flexibility of trimethylamine monooxygenase, successfully identifying variants with improved heat-resistant NADPH activity while revealing the persistent challenge of maintaining NADH function under thermal stress.
This study demonstrates that repurposed small-molecule inhibitors targeting metalloproteinases and phospholipases effectively neutralize a diverse range of venoms from the Neotropical *Bothrops* snake genus, suggesting their potential as valuable early interventions for snakebite treatment.
This study develops and validates a novel Real-time Quaking-Induced Conversion (RT-QuIC) assay to detect misfolded insulin, demonstrating its potential as a tool for investigating insulin aggregation's role in metabolic diseases and aging.
This study identifies METTL7B as a critical regulator of lipid metabolism and tumor progression in pancreatic cancer, where it is transcriptionally upregulated by HNF4A and HNF4G to promote lipid droplet accumulation, cancer cell malignancy, and poor patient prognosis.
This study demonstrates that CRISPR/Cas9-mediated editing of the conserved TaIPA1 and TaTB1 regulatory module in hexaploid wheat successfully reshapes plant architecture by significantly increasing tiller number and grain weight, offering a promising strategy for developing high-yielding wheat varieties.
This paper proposes adapting a Poisson-multinomial model for quadplexed droplet digital PCR (ddPCR) to provide a high-throughput, low-input method for screening and characterizing the integrity of viral and non-viral gene therapy vectors, while also outlining the model's limitations and best practices.
Protenix-v2 is a robust biomolecular modeling system that significantly advances high-accuracy structure prediction and design, achieving record-breaking success rates in antibody-antigen modeling, GPCR target discovery, and diverse therapeutic applications like SARS-CoV-2 binder design.
This paper presents a novel method that utilizes BstFL DNA polymerase to perform RNA-templated gap filling and ligation, enabling simultaneous whole-transcriptome and targeted mutation profiling within single cells.
By combining cryo-focused ion beam milling with cryo-electron tomography, this study reveals that ER stress in *S. cerevisiae* induces organelle remodeling and a modest increase in inactive 80S ribosomes bound to elongation factors, demonstrating that translational inhibition serves as a mechanism to alleviate ER stress in this yeast.
This study demonstrates that cryo-EM data encodes a physically grounded, probabilistic latent conformational landscape that not only aligns with independent molecular dynamics simulations and reveals biologically meaningful state transitions but also enables improved structural reconstruction through probability-guided particle selection.
This study demonstrates that neuron-specific epigenetic repression of Cdk5 via CRISPR/dCas9-HDAC3-mediated histone deacetylation impairs hippocampal-dependent fear and spatial memory in both male and female mice, revealing a critical, sex-independent role for Cdk5 acetylation in memory formation.
By integrating single-cell and spatial transcriptomics of human lung tissue with cultured fibroblast profiling, this study identifies CTHRC1+ fibroblasts as key pro-fibrotic signaling hubs in idiopathic pulmonary fibrosis while revealing that standard in vitro culture conditions significantly distort native fibroblast identity and diversity.
This study identifies the 60S ribosomal protein RPL38 as a critical regulator of start-codon stringency and reading frame selection, demonstrating that its depletion selectively suppresses canonical AUG translation while enhancing pathological C9ORF72 RAN translation by altering 60S ribosomal subunit homeostasis.
This study demonstrates that rational design of G-quadruplex ligand-oligonucleotide conjugates, particularly through optimizing oligonucleotide length, backbone composition, and sequence complementarity, enables selective and potent site-directed G-quadruplex stabilization via a cooperative dual-recognition mechanism.
This study utilizes ITS2, COI, and COII molecular markers to reveal that the *Anopheles tessellatus* complex in Indonesia comprises three distinct genetic lineages across five islands with high haplotype diversity and potential speciation in specific populations, highlighting the need for further research to clarify its role in malaria transmission.