Label-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure?
This study resolves the "10^5 gap issue" in intracellular thermal biology by demonstrating via label-free mid-infrared photothermal microscopy that cells possess water-like thermal conductivity, suggesting that the large temperature heterogeneity observed with fluorescent nanothermometers actually reflects slow, non-conductive intracellular processes rather than true thermodynamic temperature differences.