Development and validation of a 28S rDNA-based TaqMan real-time PCR assay for sensitive detection of Rhizoctonia theobromaein cassava tissues
This study developed and validated a highly sensitive and specific TaqMan real-time PCR assay targeting the 28S rDNA of *Rhizoctonia theobromae*, enabling reliable detection of the pathogen in both symptomatic and asymptomatic cassava tissues to support improved surveillance and management of Cassava Witches' Broom Disease.