388 papers
This study reveals that *Actinoplanes teichomyceticus* produces ferrioxamine-type siderophores with unexpected, metal-dependent antibacterial activity against Gram-positive bacteria, expanding their known biological role beyond iron acquisition.
This study elucidates a DltE-DltD-DltX interaction network in *Lactiplantibacillus plantarum* that coordinates the extracellular D-alanylation of lipoteichoic acids, a process essential for the bacterium's ability to promote *Drosophila* growth.
This study demonstrates that healthy sedentary adults exhibit a distinct metabolic phenotype characterized by significantly reduced mitochondrial bioenergetics and function, which can be effectively detected non-invasively through cardiopulmonary exercise testing (CPET) markers such as fat oxidation and blood lactate responses.
This study identifies PmcY (PA3962) as a periplasmic Zn2+-metallochaperone in *Pseudomonas aeruginosa* that couples Zn2+ transport to the CzcS/CzcR signaling pathway to downregulate the OprD porin, thereby reducing imipenem susceptibility while the cytosolic zinc quota remains sufficient.
This study employs a chemical genetics system to demonstrate that while some deubiquitylases (DUBs) are susceptible to targeted degradation via PROTACs or molecular glues, others resist turnover either through auto-deubiquitylation or by being inherently poor proteasome substrates.
Using magic-angle spinning NMR spectroscopy, this study reveals that the adapter protein NUB1L binds to the N-domain of the ubiquitin-like modifier FAT10 to induce intermolecular β-sheet formation and stabilize an unfolded state, thereby acting as a holdase to facilitate FAT10-mediated proteasomal degradation during inflammation.
Using time-resolved small-angle X-ray scattering combined with active site mutants and state-specific nanobodies, this study elucidates the kinetic and structural dynamics of the TM287/288 heterodimeric ABC transporter, successfully capturing a previously elusive transient fully-occluded state within its ATP-driven conformational cycle.
This study reveals that the cofactor Faf1 accelerates p97-mediated unfolding of ubiquitinated proteins by using its C-terminal UBX domain to stabilize the Ufd1-Npl4 heterodimer, thereby promoting the engagement and unfolding of the initiator ubiquitin.
This study reveals that actin filaments from two evolutionarily distant yeast species exhibit significantly faster disassembly and inorganic phosphate release compared to mammalian actin due to the absence of histidine 73 methylation, while also displaying distinct biochemical and mechanical differences between the yeast species themselves.
This study characterizes the tetrameric structure and disulfide-dependent stability of the *M. tuberculosis* Mam1A protein and demonstrates its stable interaction with Mam1B-1D and LucA to form Mam1ABCD and Mam1ABCD-LucA complexes, providing critical insights into the organization of these essential lipid uptake regulators for potential anti-TB drug development.
This study elucidates the structural and biophysical basis of *Mycobacterium tuberculosis* MfpD, revealing its dimeric Roadblock/LC7-like fold and proposing a noncanonical Switch I-dependent mechanism by which it regulates MfpB GTP hydrolysis to contribute to fluoroquinolone resistance and pathogenesis.
MetaReact is a novel, end-to-end Transformer-based model that unifies the prediction of metabolic enzymes, sites of metabolism, and major metabolites through structure-aware encoding and chemistry reaction-based pretraining, significantly outperforming existing methods to advance rational drug design and safety assessment.
This study utilizes the PRISMA technique combined with quantitative mass spectrometry to map high-resolution interaction surfaces of the cancer-associated protein ARID1A, successfully recapitulating known bindings while identifying novel interactors like TOX4, SIN3A, CDK2, and CCNA2, and revealing a new regulatory mechanism involving CDK2 phosphorylation that impacts cell proliferation.
This study elucidates the molecular mechanism by which the von Willebrand factor A-like domain 2 of collagen VII recognizes a Met-Gly-{Phi} motif on fibrillar collagens through specific hydrophobic interactions, thereby facilitating the transient recruitment of collagen I and III into interlaced anchoring fibrils essential for dermal-epidermal stability.
This study introduces MT-DS, a novel fluorescent probe that directly visualizes microtubule lattice damage in real time, revealing intrinsic defects at annealing sites and demonstrating that kinesin-1 actively generates new lattice damage during motility.
This study utilizes single-nucleus RNA sequencing to define the unique metabolic and inflammatory hepatopathy in pulmonary arterial hypertension, revealing a distinct Warburg-like phenotype, impaired endothelial barrier signaling, and activated hepatic stellate cells that correlate with disease severity.
This study demonstrates that engineering a CBL mutant to mimic SLAP2-mediated activation enhances EGFR ubiquitination and degradation, thereby attenuating tyrosine kinase signaling, and validates a small-molecule screening approach to pharmacologically target this mechanism for therapeutic downregulation of tyrosine kinase pathways.
This study utilizes AlphaFold2 predictions and experimental validation to identify a distinct clade of constitutively active, monomorphic RfaH homologs associated with virulence operons, thereby supporting a stepwise evolutionary model where RfaH specialized from an active NusG-like ancestor into an autoinhibited fold-switching regulator.
This study profiles the metabolomes of six plant callus cultures and introduces a novel pipeline, Metabolite2Function (M2F), to systematically annotate and rank their bioactive compounds for potential applications in human health and cosmetics.
This paper introduces Peptergent, a novel class of amphipathic peptides that enable the detergent-free extraction and purification of membrane proteins into stable, water-soluble assemblies, thereby overcoming traditional detergent limitations and facilitating direct structural and mass spectrometry analysis for improved proteomic coverage and drug screening.