277 papers
Biochemistry explores the intricate chemical processes that power life, bridging the gap between biology and chemistry to explain how molecules like proteins and DNA function within living cells. This field reveals the molecular machinery behind everything from metabolism to genetic inheritance, turning complex biological mysteries into understandable chemical interactions.
On Gist.Science, we bring you the freshest discoveries in this dynamic area directly from bioRxiv. Our team processes every new preprint uploaded to the server, transforming dense academic findings into both clear, plain-language overviews and detailed technical summaries. This dual approach ensures that whether you are a curious beginner or a seasoned researcher, you can grasp the significance of these breakthroughs immediately.
Below are the latest papers in biochemistry, complete with our curated summaries to help you navigate the cutting edge of molecular science.
The study demonstrates that a full-atom MPNN-based design framework (FAMPNN) successfully engineered thermostable variants of the plant dehydrogenase SrBDH1 with up to 10°C enhanced stability and extended half-life, while fully preserving its stereoselectivity for (+)-borneol.
This study utilizes a high-density genomic SNAP DNA-binding array to comprehensively define the sequence specificity, dimeric binding mechanics, and flanking sequence influences of the transcription factor GRHL2, thereby distinguishing its direct DNA occupancy from indirect cofactor-mediated recruitment.
By integrating bulk and single-cell transcriptomic analyses, this study identifies seven vascular aging-related hub genes (including TNF, MMP9, and APOE) as key diagnostic biomarkers and immune drivers in atherosclerosis, while proposing curcumin as a potential therapeutic agent targeting these genes.
This study reveals that the accumulation of native entanglement misfolding in globular proteins is a key driver of age-associated structural changes across the *Saccharomyces cerevisiae* proteome, as these entangled regions are significantly more prone to forming long-lived, near-native misfolded states during aging.
This study demonstrates that phosphorylation of the intrinsically disordered N-terminal motif A in SIRT1, particularly at Ser27, induces structural folding into an ordered conformation that significantly enhances the enzyme's deacetylase activity.
This paper proposes a revised theoretical framework that expands the classification boundaries of cephalopod reflectin proteins to accommodate newly discovered sequence diversity across ten species, enabling more accurate identification and deeper structural-functional analysis of these dynamic optical systems.
This study identifies a novel stress-response pathway in *Mycobacterium tuberculosis* where the enzyme Rv1722 synthesizes the metabolite GABA-trehalose from accumulated GABA and trehalose under hypoxic and nitric oxide stress, representing a previously unknown metabolic adaptation to immune-imposed conditions.
This study combines X-ray crystallography and molecular dynamics simulations to reveal that the turnover of carbapenem antibiotics by OXA-48 -lactamase is regulated by a complex interplay of acyl-enzyme tautomerisation, conformational dynamics, and hydration, with the OXA-519 variant showing enhanced hydrolytic potential due to a single active-site substitution.
Selective activation of estrogen receptor beta (ER{beta}) using the agonist DPN prevents lipotoxicity and restores metabolic homeostasis in estrogen-deficient models by coordinating liver lipid remodeling, enhancing fatty acid oxidation, and improving glucose and lipid profiles.
This study identifies and characterizes flavophos, a novel phosphonate antibiotic produced by *Burkholderia* that inhibits lumazine synthase, through the heterologous expression of its biosynthetic gene cluster and the structural elucidation of a divergent DUF849 enzyme involved in its synthesis.