388 papers
This study utilizes long-read genome sequencing and AlphaFold3 structural modeling to overcome assembly challenges posed by repetitive sequences, successfully identifying and characterizing 35 distinct RTX adhesins across seven Gram-negative bacterial species to inform future strategies for blocking bacterial colonization and infection.
This study optimizes the expression and purification of human STEAP1 to preserve its functional homotrimeric conformation and cofactor binding, demonstrating that stable expression systems yield superior protein quality compared to transient systems for facilitating therapeutic discovery.
By combining deuterium oxide labeling with high-resolution lipidomics, this study reveals that myelin renewal occurs through the heterogeneous, class-dependent turnover of individual lipid constituents and that this essential renewal process significantly slows with ageing and is impaired by ApoE deficiency.
This study reveals that the bacterial *alx* riboswitch integrates manganese sensing with cytoplasmic alkalinity by using pH-dependent conformational changes in a specific RNA loop to allosterically modulate metal binding affinity, thereby enabling combinatorial environmental sensing during stress.
This study demonstrates that microbes can utilize non-enzymatic, geochemically driven oxidative pathways involving light and heat to assimilate dimethyl sulfide and dimethyl sulfoxide for growth, challenging the assumption that such metabolic processes strictly require enzymatic reduction.
This study reveals that host and microbial glycine conjugation of indole-3-carboxylates generates bioactive metabolites, such as IAA-Glycine, which function as direct human Ah receptor ligands, thereby establishing a novel interkingdom link between plant-derived auxin chemistry and human physiology.
This study utilizes MALDI mass spectrometry imaging to demonstrate that aging delays osteochondral fracture healing in mice by impeding the spatial progression from cartilaginous soft callus to hardened bone callus, as evidenced by the retention of cartilage-specific proteins and the failure to upregulate bone-specific collagens in aged tissues.
This study reveals that high-affinity ligand interactions with amyloid fibrils occur via two distinct topological binding modes, a finding that enables the mathematical differentiation of these modes and guides the rational design of improved ligands with enhanced affinities and specific fluorescence responses.
This study utilizes multi-omic analyses in mice to demonstrate that the gut microbiota uniquely modulates how dietary saturated, monounsaturated, and polyunsaturated fatty acids influence host lipid metabolism and inflammatory mediators, thereby revealing critical metaorganismal crosstalk that impacts cardiometabolic disease.
By elucidating the distinct co-receptor mechanisms (LRP1 and betaglycan) of the parasite-derived TGFβ antagonist TGM6, researchers rationally engineered programmable, cell-type-specific TGFβ modulators that can selectively antagonize signaling in target cells expressing specific co-receptors.
This study demonstrates that structural modifications to oxazolidinone antibiotics, such as tedizolid and delpazolid, alter their context-specific translation inhibition by changing their nascent peptide stalling preferences and inducing a distinct compacted, helical conformation of the peptide within the ribosome.
Biochemical experiments and computational analyses demonstrate that the avian magnetoreception candidate ErCRY4a lacks the binding sites for antenna chromophores 8-HDF and MTHF, indicating it functions with a flavin-only cofactor state distinct from most photolyases.
AlphaFast is a GPU-accelerated framework that integrates MMseqs2 to drastically reduce the runtime and cost of AlphaFold 3 predictions while maintaining structural accuracy.
By introducing proline residues to disrupt -sheet formation in the FUS prion-like domain, researchers demonstrated that preventing aggregation and liquid-to-solid transitions can eliminate neurotoxicity in disease models while preserving the protein's essential phase separation and biological functions.
This study reconstitutes Ras-PI3Kγ signaling on supported membranes using light-induced inputs to demonstrate that while global inhibitors normally suppress activity, positive feedback loops enable local amplification and the propagation of activity waves, revealing that diffusion coefficients and feedback architecture are critical determinants of spatiotemporal signaling dynamics.
This study presents the first cryo-EM structure of human TRPM1 in a conducting state, revealing an unexpected clockwise domain-swapped pore architecture with a wide intracellular gate that explains its constitutive activity and provides a structural basis for understanding congenital night blindness.
This study combines structural biology and protein engineering to modify the cytochrome P450 enzyme AgcA, enabling it to efficiently O-demethylate both 4-propylguaiacol and 4-propylsyringol, thereby facilitating the biocatalytic valorization of hardwood lignin-derived aromatics.
By developing the GATO-seq assay and utilizing cryo-EM, this study reveals that a specific nucleic acid sequence induces a unique, TFIIIS-resistant "super pause" in RNA polymerase II, which stabilizes a reversible, single-nucleotide backtracked "sidetracked" state through a threonine-lined pocket, thereby demonstrating how sequence directly encodes transcriptional pausing control.
This study deciphers and demonstrates the engineerability of a structural code governing the picomolar-affinity, sequence-specific CRA-CRA pairing that dictates the precise assembly of GID/CTLH-type E3 ligases, enabling the reprogramming of their subunit interactions.
This study introduces highly sensitive and rapid chemiluminescent probes that selectively target the ClbP enzyme, enabling the direct detection of colibactin-producing *E. coli* in complex stool samples for applications in cancer research and clinical screening.