375 papers
This study comprehensively characterizes twelve ferredoxin isoforms in *Synechocystis* sp. PCC 6803 through spectroscopic, electrochemical, and expression analyses, revealing a diverse suite of proteins with varying redox potentials and regulatory patterns that collectively maintain cellular redox homeostasis by supporting both core photosynthetic electron transfer and specialized metabolic functions.
This study demonstrates that substituting tryptophan 99 with p-azido-L-phenylalanine in PrPC enables the efficient propagation of infectious prions that retain site-specific click chemistry reactivity, thereby facilitating the targeted biochemical and biological investigation of prion infectivity.
This study introduces a novel, rapid, and sensitive semi-quantitative HPLC-MS/MS method capable of detecting the widest range of isoprenoid quinones to date, successfully revealing stage-specific microbial community shifts in wastewater treatment samples.
This study reveals that the mitotic spindle regulator Mud undergoes a time-dependent liquid-to-solid phase transition via self-interacting oligomerization, a process that is prevented by phosphorylation and is also observed in other coiled-coil spindle proteins, highlighting a novel biophysical mechanism for regulating spindle-associated condensates.
This study reveals that dihydroxy-enone-type protein-bound ceramides, rather than the previously studied epoxy-enone types, are the predominant class in human stratum corneum and are likely generated via epoxide hydrolase EPHX3-mediated conversion, highlighting a fundamental structural difference between human and mouse skin barrier lipids.
This study presents the first crystal structures of the *Staphylococcus aureus* QacA multidrug transporter in multiple conformational states, revealing how ligand-induced flexibility and proton-coupled electrostatic changes drive substrate recognition and extrusion to provide a mechanistic framework for combating antimicrobial resistance.
This study elucidates the structural and mechanistic basis by which the Pseudomonas aeruginosa response regulator BqsR directly senses bioavailable ferrous iron via a His-rich motif to regulate the feo operon and control iron acquisition, representing a novel regulatory mode among OmpR-like proteins with potential therapeutic implications.
This paper presents a detailed protocol for using the automated Spitrobot crystal plunger to perform cryo-trapping time-resolved crystallography, enabling researchers to map enzymatic reaction pathways in the millisecond domain with reduced technical complexity and broader access to synchrotron facilities compared to room-temperature methods.
This study reports the development of a [13C6]scyllo-inositol isotopic tracer and demonstrates through lipidomics and NMR that scyllo-inositol is incorporated into phosphatidylinositols in mammalian cells, revealing a previously underappreciated metabolic role for this isomer.
This systematic review and indirect comparison meta-analysis concludes that current evidence is structurally insufficient to reliably compare the metabolic effects of NMN and NR due to systematic asymmetries in dosing, population demographics, and assay methods, necessitating future head-to-head trials with harmonized protocols to generate interpretable comparative data.
This study presents the first detailed steady-state kinetics analysis of *Candida albicans* Gpi8, revealing that Mn²⁺ enhances enzymatic activity by stabilizing a flexible loop for optimal substrate positioning, while demonstrating that substrate binding is length-dependent and hindered by bulky residues at the cleavage site.
This study demonstrates a highly sensitive and selective conical SiO2 nanopore biosensor capable of detecting heart-type fatty acid binding protein (H-FABP) at attomolar concentrations via ion current rectification, featuring a detection limit of approximately 0.4 aM and the ability to be regenerated for repeated use.
Time-resolved cryo-EM studies of *Mycobacterium tuberculosis* isocitrate lyase 2 reveal that acetyl-CoA binding drives asymmetric, half-of-site activation through a conformational selection mechanism that shifts the enzyme's pre-existing equilibrium toward an active state.
All-atom molecular dynamics simulations reveal that while anionic SDS induces thermal denaturation of the cationic Trp-cage miniprotein through strong hydrophobic insertion, cationic CTAB partially protects the protein from unfolding by providing a structured hydrophobic environment that stabilizes its native state.
This study demonstrates that flash-freezing RhoGEF proteins leads to unpredictable and significant losses in catalytic activity and increased data variability within a week, despite preserved thermostability and global conformation, highlighting the need for careful, protein-specific characterization of freezing effects before using these enzymes in research or therapeutic development.
This study identifies eight small molecules that robustly activate the *Staphylococcus aureus* ClpC/ClpP protease by targeting two specific regulatory sites in its N-terminal domain, thereby establishing the enzyme as a chemically targetable drug candidate for combating bacterial virulence and stress resistance.
NPannotator is an automated, genome- and chemistry-constrained pipeline that elucidates type I polyketide synthase pathways by inferring catalytic gene ordering and acyltransferase substrate specificities through iterative cheminformatics matching against a database of synthetic polyketide backbones.
This study elucidates the hierarchical physicochemical mechanism by which the cytosolic iron-sulfur cluster assembly targeting complex (CTC) recognizes diverse C-terminal tripeptide motifs through a specific aromatic pocket and hydrophobic groove at the Cia1-Cia2 interface, thereby defining the molecular grammar for selective Fe-S protein maturation.
This study identifies endogenous nucleophilic metabolites, such as taurine and spermidine, as direct scavengers of reactive acyl species that neutralize carbon stress, thereby preventing aberrant protein acylation and extending lifespan in model organisms.
This study demonstrates that the phase separation behavior of TDP-43 dictates its protein interactome and alternative splicing regulation, where distinct condensation states differentially recruit splicing factors and helicases to modulate RNA and protein homeostasis.