277 papers
Biochemistry explores the intricate chemical processes that power life, bridging the gap between biology and chemistry to explain how molecules like proteins and DNA function within living cells. This field reveals the molecular machinery behind everything from metabolism to genetic inheritance, turning complex biological mysteries into understandable chemical interactions.
On Gist.Science, we bring you the freshest discoveries in this dynamic area directly from bioRxiv. Our team processes every new preprint uploaded to the server, transforming dense academic findings into both clear, plain-language overviews and detailed technical summaries. This dual approach ensures that whether you are a curious beginner or a seasoned researcher, you can grasp the significance of these breakthroughs immediately.
Below are the latest papers in biochemistry, complete with our curated summaries to help you navigate the cutting edge of molecular science.
This paper presents a computationally designed protein binder strategy that targets the autoregulatory interface between the DHX9 helicase core and its dsRBD2 domain to specifically inhibit DHX9 activity for cancer therapy, offering an efficient alternative to traditional library-based screening methods.
This paper resolves the controversy surrounding Cooperativity definitions in PROTACs and molecular glues by demonstrating that the apparent negative Cooperativity observed in "partial-embedded" models is actually an entropic penalty from restricting the exposed mass of large ligands, thereby revealing that true positive Protein-Protein Interaction (PPI) Cooperativity exists once this ligand entropy cost is subtracted.
This paper introduces Quantal PCR (quPCR), a novel calibrator-free method that determines absolute nucleic acid copy numbers at the single-molecule level by deriving an intrinsic quantal unit from the system's thermodynamic and kinetic properties.
This study utilizes comprehensive proteomic and glycomic analyses to reveal that Influenza A virus infection significantly remodels the host glycoproteome by inducing widespread desialylation via viral neuraminidase and disrupting Golgi glycoprotein flux, while simultaneously triggering a prolonged unfolded protein response that increases oligomannose glycosylation.
This study utilizes glycoproteomics, cryo-EM, and surface plasmon resonance to demonstrate that the N- and O-linked glycan shields of alphaherpesvirus gB proteins, particularly their sialic acid content, differentially modulate binding to host co-receptors MAG, PILR, and NMHC-IIA, thereby explaining the distinct tropism and infectivity patterns among HSV-1, HSV-2, and VZV.
This paper introduces an iterative sampling framework that leverages coevolutionary information and residue-specific frequencies to efficiently generate diverse, high-quality ensembles of distinct protein conformations, significantly outperforming existing methods in characterizing the structural landscapes of fold-switching proteins.
This study demonstrates that dietary cholesterol reduces systolic blood pressure and attenuates renal inflammation in stroke-prone spontaneously hypertensive rats by altering lipid profiles and suppressing the arachidonic acid-prostaglandin E2 axis, thereby providing a potential mechanistic explanation for its previously observed lifespan-extending effects in this model.
This study reveals that the predatory bacterium *Bdellovibrio bacteriovorus* utilizes a unique double-TULIP protein, TipA, which forms a lipid-sequestering homodimer that is structurally opened by its adapter protein TipB, representing the first example of an adaptor-mediated conformational change in TULIPs.
This study characterizes ulacamten as a novel cardiac myosin inhibitor with a distinct regulatory light chain-dependent mechanism that effectively improves diastolic function and reduces cardiac hypertrophy in preclinical models of heart failure with preserved ejection fraction (HFpEF).
This study reveals that the SARS-CoV-2 helicase Nsp13 functions as a multifaceted, tunable nucleic acid remodeler that integrates ATP-dependent motor activity with Mg²⁺-primed, ATP-independent remodeling, strand annealing, and chaperone functions to dynamically adapt to diverse structural demands during viral replication.