316 papers
Biochemistry explores the intricate chemical processes that power life, bridging the gap between biology and chemistry to explain how molecules like proteins and DNA function within living cells. This field reveals the molecular machinery behind everything from metabolism to genetic inheritance, turning complex biological mysteries into understandable chemical interactions.
On Gist.Science, we bring you the freshest discoveries in this dynamic area directly from bioRxiv. Our team processes every new preprint uploaded to the server, transforming dense academic findings into both clear, plain-language overviews and detailed technical summaries. This dual approach ensures that whether you are a curious beginner or a seasoned researcher, you can grasp the significance of these breakthroughs immediately.
Below are the latest papers in biochemistry, complete with our curated summaries to help you navigate the cutting edge of molecular science.
This paper investigates how combining two antagonistic integral controllers at a shared variable creates distinct regulatory modes—delegated, isolated, and metastable control—enabling both homeostatic and rheostatic behaviors, with applications explaining photoperiodic weight changes in Siberian hamsters and the complex dynamics of blood glucose homeostasis.
This study uses all-atom molecular dynamics simulations to reveal that while tetraethylammonium blocks Kv2.1 channels by binding on-axis near the selectivity filter, the specific inhibitor RY785 binds off-axis to the channel walls, stabilizing a semi-open gate state that occludes ion flow and influences distant voltage sensors.
This study validates a high-throughput ELISA assay demonstrating that the drug lumacaftor selectively inhibits von Willebrand factor binding under inflammatory, oxidizing conditions, offering a promising strategy for developing anti-thrombotic therapeutics that minimize bleeding risks.
This study demonstrates that Xer recombination at the plasmid pSC101 psi site proceeds with a precise topological linking number change of +4, converting four negative supercoils into catenation nodes via an antiparallel alignment and Holliday junction intermediate to ensure stable plasmid inheritance.
This study resolves crystal structures of an ASBT homologue and a humanized version to reveal that, unlike NTCP, these SLC10 family transporters lack a transmembrane pore due to a flexible TM6 helix, and molecular dynamics simulations further demonstrate how membrane lipids facilitate the transport of bile acids with large hydrophobic groups.
The paper introduces RIPUP, a rapid multi-protease workflow combining Arg-C Ultra and r-Chymotrypsin digestion with TMT labeling and HiP-Frag analysis to reduce histone PTM sample preparation from days to hours while significantly expanding coverage to detect previously elusive acylations and variant-specific modifications.
This study identifies AltSLC35A4, a conserved microprotein encoded by an alternative open reading frame, as a novel regulator of mitochondrial DNA homeostasis that interacts with actomyosin cytoskeletal components to maintain nucleoid spatial distribution and copy number independently of replication or bioenergetic states.
This study demonstrates that while multiple data analysis methods can correlate biochemical inhibition with antiviral activity for MERS-CoV main protease inhibitors, an enzyme kinetics model accounting for dimerization and biphasic concentration-response curves provides the most accurate prediction of cellular efficacy.
This study reveals that the Rpd3L histone deacetylase complex acts as a metabolic gatekeeper in *Saccharomyces cerevisiae* by dynamically removing H3K9 acetylation from active promoters upon nutrient shifts, thereby coordinating the shutdown of growth programs and ensuring transcriptional fidelity during metabolic transitions.
This study identifies 4,5,6,7-tetrabromo-1H-benzotriazole (TBB) as a specific small-molecule inhibitor of the NADPH phosphatase MESH1 that effectively suppresses ferroptosis in vitro and reduces neuronal death in an Alzheimer's disease model, validating MESH1 as a promising therapeutic target.