375 papers
This study reveals that the alpha-carboxysomal carbonic anhydrase CsoSCA is redox-regulated via a conserved vicinal cysteine pair, where oxidation induces global conformational changes that activate the enzyme to prevent cytosolic short-circuiting of the CO2-concentrating mechanism.
This study demonstrates that attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, combined with regression modeling, enables rapid, label-free, and non-destructive global quantification of DNA 5-methylcytosine and 5-hydroxymethylcytosine modifications, including in circulating tumor DNA samples.
This study demonstrates that self-assembled nucleolipid-modified G-quadruplexes (NLG4) effectively suppress pancreatic cancer by acting as multitarget decoys that downregulate key oncogenes (KRAS, MYC, KIT, BCL2), inhibit pro-proliferative signaling pathways, and potentiate gemcitabine efficacy through enhanced cellular internalization and interaction with G-quadruplex unfolding factors.
This study combines site-directed spin labeling and DEER spectroscopy to reveal that while inhibitors trap the transducin-bound phosphodiesterase-6 complex in an inactive conformation, the simultaneous binding of substrate and transducin induces a highly dynamic active state consistent with an alternating-site catalytic mechanism.
This paper presents optimized protocols for using fluorescent D4 probes (D4-mCherry and D4-GFP) to simultaneously achieve accurate quantification and spatial visualization of membrane-accessible cholesterol in cells while preserving the native membrane environment.
This study identifies and characterizes thermolanthin, a novel class II lanthipeptide from the thermophilic bacterium *Thermoactinomyces* sp. DSM 45891, which features unique DL-(methyl)lanthionine ring structures deviating from typical stereoselectivity and exhibits antimicrobial activity against Gram-negative ESKAPE pathogens.
This study reports the total chemical synthesis and structural characterization of a novel, highly stable disulfide-rich miniprotein scaffold from the snail *Biomphalaria glabrata*, revealing a new protein fold and establishing a framework for its functional exploration and potential applications.
This study reveals that the lipolytic enzyme ATGL promotes genomic stability by facilitating DNA repair through lipid droplet-mediated bulk acetylation of chromatin-bound proteins, thereby reducing DNA damage burden and cellular senescence.
This study identifies TvAPT, a cyanobacterial adenine prenyltransferase with an enlarged binding pocket that enables the unprecedented N6-prenylation of adenine substrates using extended C10 and C15 prenyl donors, thereby expanding the chemical space for biomolecular engineering.
This paper presents a single-molecule nanopore sensing strategy using RNA:DNA nanostructures to directly quantify and discriminate disease-associated RNA tandem repeat expansions with 18-nucleotide resolution, offering a promising tool for diagnosing repeat expansion disorders like DM1, DM2, and CCHS1 in complex biological samples.
By developing a paired Ala-Aib helix scanning method, this study reveals that the relationship between cathelicidin helicity, membrane permeabilization, and bacterial killing is species-specific rather than universal, enabling the design of more selective antimicrobial compounds and challenging the prevailing model that helical structure and membrane disruption are strictly required for bacterial killing.
This study identifies CKAP2 as a direct substrate of the Aurora A/TPX2 complex, demonstrating that Aurora A-mediated phosphorylation of CKAP2 reduces its microtubule affinity to regulate mitotic spindle growth and stability.
This study utilizes quantitative proteomic and metabolomic profiling of OAT-deficient mice to reveal that while the liver compensates for ornithine accumulation through enhanced urea cycle activity, the eye—specifically the RPE/choroid—exhibits extensive early molecular disruptions in mitochondrial metabolism, cytoskeleton, and antioxidant capacity that explain its selective vulnerability in gyrate atrophy.
This study reveals that bacterial thioester domains (TEDs) mediate a chemically encoded, pH-tunable covalent adhesion mechanism where physiological pH promotes tight binding to ligands like fibrinogen, while mild acidification triggers rapid dissociation, a conserved feature across Gram-positive bacteria.
This study reveals that PDS5A and TOP2B cooperatively recruit each other to CTCF-bound chromatin sites via a novel CTCF interaction region to maintain genome organization and regulate gene expression, with dysregulation of this axis contributing to glioma heterogeneity and influencing sensitivity to TOP2-targeted cancer therapies.
This study presents a high-resolution (1.43 Å) crystal structure of *Helicobacter pylori* glutamate racemase in a monoclinic form, revealing that while the unit-cell parameters and crystal packing differ from previous models, the enzyme's conserved head-to-head dimeric assembly and active-site architecture remain unchanged.
This study demonstrates that unlike its *E. coli* counterpart, the *Bacillus subtilis* translesion polymerase Pol Y1 is not strongly recruited to replication sites upon various types of DNA damage, revealing significant mechanistic differences in bacterial translesion synthesis regulation.
This study presents the first high-resolution cryo-EM structure of human ATAD2B, revealing its functional hexameric architecture and biochemical activity to provide mechanistic insights into its role as a dysregulated AAA+ ATPase in disease states.
This study characterizes full-length human TBCK as a class I multidomain pseudokinase lacking nucleotide binding and canonical catalytic motifs, thereby clarifying its biochemical properties to advance the understanding of TBCK-related encephalopathy.
The researchers developed TPAsense, a stabilized protein-based biosensor capable of rapidly and accurately detecting nanomolar concentrations of terephthalate to accelerate the screening of plastic-degrading enzymes and monitor microplastic pollution.