276 papers
Biochemistry explores the intricate chemical processes that power life, bridging the gap between biology and chemistry to explain how molecules like proteins and DNA function within living cells. This field reveals the molecular machinery behind everything from metabolism to genetic inheritance, turning complex biological mysteries into understandable chemical interactions.
On Gist.Science, we bring you the freshest discoveries in this dynamic area directly from bioRxiv. Our team processes every new preprint uploaded to the server, transforming dense academic findings into both clear, plain-language overviews and detailed technical summaries. This dual approach ensures that whether you are a curious beginner or a seasoned researcher, you can grasp the significance of these breakthroughs immediately.
Below are the latest papers in biochemistry, complete with our curated summaries to help you navigate the cutting edge of molecular science.
The NanoBridge system is a modular platform that utilizes biologic binders to recruit small protein tags to challenging, often disordered endogenous proteins, enabling precise, reversible, and multiplexed post-translational modification editing in live cells without the need for exogenous PTM writers.
This study demonstrates that substituting heme B with structurally conservative non-natural metalloporphyrins in de novo designed tetrahelical proteins enables high-affinity binding and broad, predictable tuning of redox potentials by over 400 mV without compromising protein structure, establishing a versatile platform for engineered bioenergetic and bioelectronic applications.
This study reveals that the KN domain of KANK proteins consists of separable modules, where residues 30–60 mediate talin binding and residues 60–68 drive a conserved intramolecular interaction, establishing a modular architecture that supports an autoinhibitory regulatory mechanism.
This study establishes an optimized mass spectrometry workflow for the quantitative profiling of JMJD6-catalysed lysine hydroxylation, revealing that this modification exhibits residue-dependent oxygen sensitivity and heterogeneity in hypoxia-responsive kinetics across bromodomain proteins.
CascadeMAP is an autonomous, closed-loop microfluidic platform that integrates Bayesian optimization and multi-agent AI to rapidly optimize complex enzyme cascades without human intervention, processing hundreds of thousands of reactions to accelerate the development of biocatalytic systems.
Despite the potent in vitro inhibition of *Plutella xylostella* glycogen phosphorylase by specific inhibitors and RNAi knockdown, the enzyme is not a viable insecticidal target because the insect effectively compensates for the metabolic block through upregulated gluconeogenesis and protein catabolism, allowing for full developmental recovery and fitness.
This study reveals that Cu/Zn superoxide dismutase (SOD1) functions as a copper-dependent thiol oxidase that generates potent thiosulfinates, thereby establishing a dual role for this enzyme in driving both cytotoxic oxidative stress and pro-growth signaling through a newly identified sulfur-based oxidation pathway.
This study utilizes cryo-EM to reveal that the Parkinsonism-associated proteins FBXO7 and PI31 independently bind to and inhibit the proteasome 20S core particle through distinct structural mechanisms, providing a molecular explanation for how disease-linked variants disrupt proteasome regulation and contribute to neurodegeneration.
This paper presents a rapid generative AI method that successfully designs programmable asymmetric -barrel nanopores with tunable dimensions and functionalities, such as ion sensing and DNA translocation, overcoming the limitations of previous uniform lumen structures and expert-dependent design approaches.
Molecular dynamics simulations reveal that deleting the central region of the cellular prion protein disrupts stabilizing intramolecular interactions, causing the N-terminal domain to adopt extended conformations and increase its proximity to the plasma membrane, thereby providing a mechanistic explanation for the neurotoxicity associated with this mutation.