382 papers
This study demonstrates that while plasmin-mediated cleavage of GPIbα disrupts platelet-von Willebrand factor complexes in vitro by reducing platelet binding capacity, its role in breaking down these complexes in vivo during thrombotic thrombocytopenic purpura appears limited.
This study demonstrates that replacing the inactivating reducing agent dithiothreitol (DTT) with tris(2-carboxyethyl)phosphine (TCEP) enables the crystallization of active human aldehyde oxidase in a new orthorhombic form, thereby overcoming previous limitations and facilitating future time-resolved crystallographic applications.
This study reveals that glutamate dehydrogenase (GDH) activity and hexameric assembly are reversibly regulated by S-palmitoylation at specific cysteine residues, which inhibits the enzyme by disrupting its quaternary structure, while mitochondrial thioesterases APT1 and ABHD10 restore its active form by removing the lipid modification.
This study establishes a human cell-free high-throughput screening platform to identify the NT-2 mycotoxin as a novel ribosomal inhibitor that binds the peptidyl transferase center and induces a dormant state in mammalian protein synthesis.
This study reveals that the R2TP complex, specifically through the PIH1D1 subunit, stabilizes p53 to regulate the cell cycle, offering a potential therapeutic strategy for restoring p53 function in cancer cells.
This study reveals that FoTO1 orchestrates early Taxol biosynthesis through a dual mechanism, acting both as a catalytic enzyme converting taxadiene-(4),5-epoxide to taxadien-5-ol and as a non-catalytic scaffold that organizes P450 enzymes to enhance metabolic flux across plastid and ER compartments.
This study elucidates the mechanism by which the bacterial TAM complex transports phospholipids to the outer membrane, revealing that TamA anchors TamB to form a stable hybrid-barrel structure with a lipid-conducting -taco channel that maintains cardiolipin levels and suggests an evolutionary link to eukaryotic lipid transfer proteins.
This study elucidates the modular architecture and molecular mechanisms of RAD54B in homologous recombination, revealing how its N-terminal domain and unique beta-domain stabilize RAD51 filaments, regulate ATPase activity, and promote strand invasion to ensure efficient DNA double-strand break repair.
This study establishes a systematic framework linking siderophore biosynthesis to primary metabolism, demonstrating that targeted supplementation of specific precursors enhances the iron-scavenging capabilities and pathogen-suppressive activity of the beneficial bacterium *Bacillus amyloliquefaciens* against *Ralstonia solanacearum*.
The authors engineered non-CpG-specific DNA methyltransferases to introduce an unnatural 5-carboxymethylcytosine modification as a bio-orthogonal label, enabling the reliable, multimodal mapping of protein-DNA occupancy alongside native epigenetic marks without confounding readouts.
This study identifies the Atlas virus family in nematodes, characterizes their unique structural and functional properties via cryo-EM, and demonstrates their potential as engineered, scalable vehicles for tissue-specific RNA delivery.
This study identifies FIKK1 as a critical kinase that phosphorylates the placental malaria adhesin VAR2CSA, thereby enhancing its binding to chondroitin sulfate A and facilitating the sequestration of *Plasmodium falciparum*-infected erythrocytes in the placenta, which highlights FIKK1 as a promising drug target for placental malaria.
This study introduces a novel cell-specific isotope labeling strategy to demonstrate that neurons transfer myo-inositol to oligodendrocyte progenitor cells via the SLC5A3 transporter to promote myelin repair, a process that can be therapeutically rescued by dietary myo-inositol supplementation during demyelination.
This study utilizes temperature-replica exchange molecular dynamics simulations to demonstrate that N-terminal chirality inversions and specific sequence variations in Amyloid-beta 42 non-locally modulate its conformational landscape and phase transition behavior, revealing that the pathogenic or protective nature of these variants correlates with their structural similarity to the wild-type ensemble.
This study combines experimental binding assays and molecular dynamics simulations to demonstrate that the SV2C-selective ligand UCB-1A maintains stable binding at physiological temperatures due to a unique hydrogen bond involving Tyr298, whereas its binding to SV2A is significantly destabilized by heat, providing a mechanistic explanation for the ligand's temperature-independent selectivity.
The paper introduces "Enriched-GF," a rapid and reproducible three-hour protocol combining glass bead agitation, freeze-thaw cycling, and calcium stimulation to produce a high-yield, standardized autologous growth factor concentrate that significantly outperforms conventional platelet lysate and growth factor concentrate methods in regenerative medicine applications.
Researchers rationally designed a cell-permeable peptide inhibitor that disrupts the interaction between oxidized PTP1B and 14-3-3ζ, thereby preventing PTP1B inactivation, enhancing its tumor-suppressive activity, and inhibiting the growth of EGFR-driven cancer cells.
This study identifies human propionyl-coenzyme A carboxylase and the PRMT5:MEP50 complex as unexpected contaminants in affinity-purified proteins from HEK293 cells, demonstrating how cryo-EM and mass spectrometry can reveal these impurities and underscoring the critical need for high resin specificity in structural biology workflows.
This study demonstrates that mutating the highly conserved isoleucine 563 in the hydrophobic gas channel of NiFe CO-dehydrogenase significantly enhances O2 resistance by altering residue flexibility and cavity size, though this improvement comes with an unavoidable trade-off that also affects CO diffusion.
This study demonstrates that structure-based docking of a 2.6 billion molecule library against the Cannabinoid-2 receptor successfully identified eight diverse, potent, and subtype-selective ligand families by targeting polar orthosteric residues, with experimental validation confirming the accuracy of the docking predictions and the efficacy of subsequent structure-based optimization.