478 papers
This paper introduces Spatial Spectral Single-Molecule Microscopy (S3M), a simplified imaging technique that utilizes commercially available color CMOS detectors to simultaneously recover molecular position and spectral fingerprints from single images without the need for complex optical beam splitting or registration.
Using NMR spectroscopy, this study elucidates the distinct roles of the three binding sites within the trivalent CpCBM92A protein, revealing how their cooperative interactions and specific preferences for glucan chain segments enable the cross-linking of scleroglucan chains.
Using cryo-ptychographic X-ray computed tomography alongside electron microscopy, this study reveals the 3D developmental stages of *Gephyrocapsa huxleyi* coccolith formation, demonstrating how intracellular confinement shapes crystal morphology and offering insights into biomineralisation pathways for advanced material design.
This paper presents a novel method that utilizes 3D single-molecule tracking data and hidden Markov modeling to accurately recover membrane interaction kinetics in rod-shaped bacteria by identifying membrane-associated motion through trajectory curvature, overcoming the limitations of conventional 2D analyses that rely on diffusion rate changes.
This study demonstrates that proton tunneling at the conserved calcium activation site of the ryanodine receptor provides a temperature-invariant stochastic component that stabilizes calcium-induced calcium release, thereby ensuring robust rhythmicity in sinoatrial node cells across physiological temperatures.
By combining pharmacological experiments on sea star oocytes with an active fluid model, this study reveals that the rate of cellular deformation is optimized by balancing the ratio of myosin motors to passive crosslinkers, which jointly regulate cortical active stress and viscosity.
This study demonstrates that intrinsic mitochondrial membrane curvature acts as a thermodynamic control parameter to drive the prewetting condensation of the transcription factor TFAM, thereby establishing a geometric principle for organizing mitochondrial nucleoids without relying solely on chemical signaling.
This study reveals that cells in a partial epithelial-mesenchymal transition (EMT) state drive fast, persistent collective migration through a unique "collision guidance" mechanism that coordinates heterogeneous cell populations and enables tissue monolayers to displace others, bridging cellular interactions to emergent tissue-level behaviors.
This study demonstrates a DNA-based functionalization strategy that renders hydrophobic lanthanide nanoparticles hydrophilic and stable under electron microscopy preparation protocols, enabling multicolor cathodoluminescence imaging for the simultaneous visualization of specific proteins and cellular ultrastructure.
This paper introduces CheMelt, an online tool for the global analysis of combined thermal and chemical denaturation data, which was used to demonstrate that highly thermostable de novo designed proteins exhibit distinct thermodynamic properties and that high thermal stability does not necessarily equate to high equilibrium stability.
This study reveals that pathogenic SNCA mutations (A53T, G51D) induce distinct left-handed Lewy fold dimer structures with twist reversal in familial Parkinson's disease and dementia, whereas the non-pathogenic H50Q variant and human A53T transgenic mouse models exhibit different filament architectures resembling wild-type or multiple system atrophy folds, respectively.
By integrating fluorescence microscopy, cryo-electron microscopy, and molecular simulations, this study reveals that arginine-rich peptides penetrate cells through a unified mechanism of membrane folding and multilamellar stacking, with observed morphological variations arising from the size of the available membrane reservoir.
This paper introduces a novel dual-branch U-Net architecture with a shared encoder and cross-attention gating, trained on synthetic microplate data, that simultaneously performs robust Raman baseline correction and denoising while enabling quantitative analysis through photon counting.
This paper proposes a hybrid deterministic-stochastic framework demonstrating that the evolutionary success of viral recombinants is governed by a non-monotonic "generation-establishment tradeoff," which creates a sharply localized temporal window of opportunity determined by the dynamic interplay between parental abundance-driven recombination and competitive suppression.
This study demonstrates that the carotenoid 4,4'-diaponeurosporenoic acid (4,4'-DNPA), a precursor to staphyloxanthin in *Staphylococcus aureus*, enhances membrane order and stability by increasing lipid packing and phase transition temperatures, thereby complementing staphyloxanthin's role in biophysically regulating the bacterial membrane.
This study elucidates the molecular mechanism by which the anti-CRISPR protein AcrIIC1 inhibits GeoCas9 by combining X-ray crystallography and dynamics analysis to reveal how binding at the HNH catalytic site disrupts electrostatic contacts, rewires intrinsic dynamics, and reduces guide RNA affinity.
This study reveals that the *Listeria monocytogenes* prfA RNA thermometer activates virulence gene translation at host temperature through a hierarchical mechanism where localized ribosome binding site opening drives activation while a distal, persistently folded upper helix remotely tunes thermal sensitivity.
This paper introduces two new deterministic seeding strategies, strat_all and strat_reduced, for the NANI k-means clustering method that significantly accelerate the analysis of multi-million frame molecular dynamics simulations while maintaining high clustering quality and reproducibility.
This study reveals that the KcsA potassium channel achieves high-flux, ultralow energy-consumption transport through a mechanism where hydrated K+ ions undergo resonant energy transfer and tunneling-like motion to shed their hydration shells in two distinct steps without dragging water molecules into the channel.
This study presents the first time-resolved measurements of real contact area and tangential force under electrostatic actuation, revealing that finger-surface interactions are governed by competing vibration and adhesion regimes that exhibit an inverted U-shaped dependence on frequency and are significantly modulated by skin moisture.