375 papers
This study reports cryo-EM structures of the human herpesvirus 1 helicase-primase complex bound to inhibitors amenamevir and pritelivir, revealing how these drugs lock the helicase in an inactive conformation to provide a structural basis for developing antivirals against other herpesvirus subfamilies.
This study identifies and characterizes flavophos, a novel phosphonate antibiotic produced by *Burkholderia* that inhibits lumazine synthase, through the heterologous expression of its biosynthetic gene cluster and the structural elucidation of a divergent DUF849 enzyme involved in its synthesis.
This study demonstrates that while engineered {psi}-{chi} fusion proteins are biochemically active, they fail to restore AZT tolerance in *E. coli* because the drug resistance mechanism requires {chi} to dynamically interact with SSB and the YoaA helicase independently of its stable association within the DNA polymerase III clamp loader complex.
The paper introduces SpecReBoot, a statistical framework that applies bootstrap resampling to MS/MS similarity scoring to quantify edge confidence in molecular networks, thereby filtering noise and revealing hidden chemical relationships, as demonstrated by the discovery of a novel macrolactone scaffold.
This study demonstrates that in mice, plasmanyl lipids are sufficient to prevent cataracts and maintain fertility, indicating that plasmalogens are dispensable for retinal integrity and reproductive function despite their critical role in total ether lipid deficiency.
This study presents the first proteome-wide analysis revealing that mammalian proteins, including transcription factors and DNA repair enzymes, exhibit diverse and symmetry-specific recognition patterns for 5-formylcytosine modifications in CpG dyads, thereby modulating target selection and chromatin regulation.
This study elucidates how TFAM-induced promoter bending creates a transcription-stimulatory interface between PolRMT and upstream DNA, while a PolRMT tether helix acts as an autoinhibitory element to ensure promoter specificity, thereby defining the structural mechanisms governing mitochondrial transcription regulation.
This study elucidates the structural mechanism of Gi-biased allosteric modulation in the GPR84 receptor by identifying a novel helix 8-proximate binding site for the positive allosteric modulator PSB-16671, which stabilizes a specific receptor conformation to enhance macrophage phagocytosis while avoiding desensitization.
This study introduces a nanobody-based immunoprecipitation coupled with native mass spectrometry approach to directly profile endogenous protein complexes in human and mouse brain tissues, revealing region-specific mGluR2/3 heterodimer abundance that correlates with stress susceptibility and depression.
This study demonstrates that Vesicular Stomatitis Virus infection promotes the phosphorylation of UBE2J1 at serine residue S184, a modification that enhances both viral replication and cellular syncytialization, with soluble forms of the protein potentially playing an even more significant role in these processes.
This study identifies TCF25 as a predominantly cytoplasmic, membrane-associated protein that regulates the stability of GPRASP2, a relationship disrupted by the L415P variant, thereby establishing a new role for TCF25 in cellular proteostasis.
This study reveals that the BrxC AAA+ ATPase protein in Type I BREX systems regulates the balance between host genome protection and phage restriction by forming competing self-associating and heteromeric complexes whose assembly and DNA-binding activities are modulated by ATP hydrolysis and interactions with BrxB and PglZ.
This study demonstrates that a urea-gradient nanoDSF approach overcomes the kinetic stability limitations of hyperthermophilic proteins, enabling reliable determination of their melting temperatures by inducing detectable unfolding transitions that are absent under native conditions.
This paper presents an enzymatic ligation strategy that attaches short, decyl-functionalized DNA oligonucleotides to RNA fragments to significantly enhance their electrospray ionization efficiency and liquid chromatography retention, thereby enabling more sensitive mass spectrometry characterization of low-abundance RNA samples and their modifications.
This study demonstrates that three extensively engineered, hyper-stable human serum albumin variants (hSA1, hSA2, and hSA3) produced in *E. coli* retain the native protein's structural integrity, FcRn-mediated recycling, and drug-binding capabilities while offering a sustainable, animal-free alternative for biotechnological and therapeutic applications.
This study reveals that the atypical tardigrade protein RvPrxL, which has lost its canonical antioxidant functions due to a catalytic cysteine replacement, has been neofunctionalized to form a unique 20-mer complex that binds nucleic acids in the nucleus via distinct "on-ring" and "in-ring" modes.
This study reveals that the prokaryotic ligase PafA achieves broad substrate specificity for proteasomal degradation through an ensemble-driven recognition mechanism, where dynamic conformational sampling and distributed structural contacts enable geometric compatibility with diverse substrates rather than relying on conserved sequence motifs.
HyperLab is an accessible, AI-driven web platform developed by HITS that streamlines the entire structure-based drug discovery workflow through advanced tools for binding prediction, molecular design, and ultra-large-scale screening, achieving high accuracy and rapid experimental validation while significantly lowering the time, cost, and expertise barriers for researchers.
This study demonstrates that monoubiquitylated H2A.Z (H2A.Zub) functions as a global transcriptional repressor by associating with diverse silenced chromatin states, including those marked by H3K27me3, DNA methylation at CpG islands, and homopurine/homopyrimidine sequences.
This study demonstrates that machine learning models identify pH-dependent lipophilicity (logD) as the dominant factor governing small molecule partitioning into biomolecular condensates, establishing ionization-coupled partitioning as a key mechanistic driver of phase separation behavior.