388 papers
Using cryo-electron microscopy, this study reveals that the ATPase subunit ChlI of magnesium chelatase forms ATP-dependent helical filaments that undergo compaction and partial Mg2+ dehydration upon hydrolysis, creating a specific conformation required for ChlD engagement and modulated by membrane lipids.
This study reveals that the *Bacillus subtilis* extracellular matrix protein TasA forms a viscoelastic, self-healing metallogel in the presence of zinc ions, which induces a morphological transition from fibers to sheets through coordinated molecular changes, offering a novel natural matrix-mimic for biofilm research.
This study demonstrates that natural isotope abundance ({delta}{superscript 1}3C and {delta}{superscript 1}N) reflects distinct metabolic reprogramming and differential responsiveness to dichloroacetate treatment in breast cancer models, suggesting that lipid-derived {delta}{superscript 1}N could serve as a noninvasive biomarker for tumor metabolic state.
This study identifies and optimizes covalent small-molecule modulators, culminating in the compound TH-B10, which directly degrades the inflammatory transcription factor IRF8 and secondarily suppresses IRF5 activity to shut down pro-inflammatory gene expression.
This paper reports the development of serapH, a highly photostable pH biosensor based on the mStayGold scaffold, along with an efficient bacterial colony screening method, to overcome the photostability limitations of existing GFP-based sensors and enhance spatiotemporal resolution in cellular imaging.
Salmonella employs the T3SS-2 effector SseK1 to specifically arginine-GlcNAcylate the host SUMO E2-conjugating enzyme UBC9 at residue R17, sterically blocking SUMO binding and thereby dismantling the SUMOylation machinery to suppress antimicrobial defense and promote bacterial virulence.
This study presents the first prospective ICH Q2(R2)-aligned total-error validation of a label-free untargeted proteomics workflow for host cell protein quantification in biotherapeutics, demonstrating that the method achieves regulatory-grade accuracy, precision, and robustness across diverse analytical conditions with a lower limit of quantification of 20 ng.
This paper presents an improved workflow for rapid, large-scale protein production in HEK293 cells that utilizes orthogonal antibiotic selection to decouple cell enrichment from target expression, thereby generating highly homogeneous, inducible populations capable of producing complex multi-subunit assemblies within 3–4 weeks.
This study establishes the first population-scale red blood cell proteome atlas from over 13,000 donors, revealing genetically encoded molecular aging clocks that predict hemolysis, transfusion efficacy, and long-term donor activity while linking accelerated RBC aging to conditions like G6PD deficiency and iron deficiency.
This study utilizes spatial proteomics to reveal that *Paramecium bursaria* maintains its endosymbiotic relationship with green algae by remodeling host lipid droplets to accumulate near the algae, a process essential for sustaining the symbiosis.
This study demonstrates that in pancreatic β-cells, the inositol phosphate IP5, synthesized by IPMK and ITPK1, acts as a metabolic regulator that stabilizes the active mTORC1 complex to prolong signaling duration without affecting its initial activation.
This study utilizes integrated proteomic and phosphoproteomic profiling to demonstrate that Bisphenol-A induces placental toxicity in extravillous trophoblast cells by dysregulating specific signaling cascades, particularly through the altered phosphorylation of c-JUN and GSK3, thereby threatening feto-placental health.
This paper introduces CLIP-tag2, an engineered protein tag paired with an optimized substrate that achieves nearly 1000-fold faster labeling kinetics and high efficiency in live cells, enabling rapid, specific, and multiplexed fluorescence imaging.
This study presents the first high-resolution cryo-EM structure of the inactive adhesion GPCR ADGRV1, revealing that its unique structural features, including a divergent tethered agonist and a closed intracellular loop 3, enable weak constitutive Gi signaling through an activation mechanism distinct from the canonical tethered agonist model.
This study reports the development of a metabolically stabilized, trans-cyclobutane-based covalent handle targeting the E3 ligase RNF126, which overcomes the cytotoxicity of previous fumarate-based designs to enable the rational creation of potent molecular glue degraders for transcriptional regulators, including the undruggable AR-V7 variant.
This paper introduces the first fully automated HDX-MS platform featuring a two-stage delipidation workflow that enables the characterization of membrane protein dynamics, such as those of the ABC transporter MsbA, within their native lipid environments, revealing physiologically relevant motions obscured by traditional detergent-based methods.
This study reveals that tyrosine phosphorylation of the C-terminal helix of PI4KA directly inhibits its lipid kinase activity without disrupting membrane recruitment, representing an evolutionarily conserved regulatory mechanism across PI3Ks and PI4Ks.
This paper presents and validates a comprehensive bioinformatics pipeline for analyzing high-throughput SELEX data, which was used to characterize the DNA-binding preferences of human and *C. elegans* POT1 homologs, revealing their affinity for G-enriched sequences and, in the case of *C. elegans* POT-1, secondary structural elements.
This study elucidates the molecular mechanism of Nar1 recruitment to the cytosolic iron-sulfur cluster assembly targeting complex (CTC), revealing that a bipartite interaction involving both a primary electrostatic anchor on Cia1 and a secondary peptide binding at the Cia1-Cia2 interface positions Nar1 for efficient Fe-S cluster transfer.
This study compares natively shed extracellular vesicles (EVs) and mechanically derived vesicles (MVs) from HER2-overexpressing SKBR3 cells, concluding that MVs offer a more suitable platform for determining the structure of membrane proteins in their native membrane environments due to their superior structural uniformity compared to the highly diverse EVs.