388 papers
This study identifies functional CXCL17 orthologs in cartilaginous fishes through integrated genomic and experimental approaches, demonstrating their high-affinity interaction with GPR25 and establishing that the CXCL17-GPR25 signaling pathway originated in ancient cartilaginous fish ancestors or earlier.
This study systematically evaluates how fungal selection strategies, phylogenetic classification, and environment-mimicking culture conditions influence chemical diversity in natural product libraries, providing practical guidelines to optimize library design for more efficient drug discovery.
This study demonstrates that inducing bleb formation during mRNA-LNP preparation using high ionic strength acidic citrate buffers significantly enhances liquid shelf-life by reducing mRNA degradation and lipid-mRNA adduct formation, offering a universal, chemistry-independent strategy to enable extended refrigerated storage without modifying the mRNA or lipid components.
This study identifies the non-coding RNA RsaE as a critical regulator in MRSA that, in conjunction with RNase Y, fine-tunes phenol-soluble modulin (PSM) expression through post-transcriptional and transcriptional mechanisms, thereby controlling biofilm architecture, cell viability, and systemic dissemination during infection.
This study reveals that the atypical kinase-like protein UbiB functions as an ATP-dependent decarboxylase in bacterial ubiquinone biosynthesis, providing an alternative decarboxylation mechanism that operates independently of the previously known UbiX/UbiD system.
This study establishes N6-methyldeoxyadenosine (N6medA) as a novel, age-associated DNA modification in the human brain that accumulates linearly with chronological age, shows elevated levels in Alzheimer's disease, and interacts with specific reader proteins to potentially drive neurodegenerative processes.
This study demonstrates that cancer-associated MUTYH variants within the interdomain connector exhibit differential functional impacts, with some impairing lesion recognition and others disrupting downstream repair steps, highlighting the necessity of combining in vitro and cellular assays for accurate variant classification.
This study reveals that host-derived N-glycosylation of the Mycobacterium tuberculosis Ag85B antigen in nucleic acid vaccines creates a physical and biochemical shield that occludes immune epitopes and recruits inhibitory Siglec-9 receptors, thereby explaining the vaccine's lack of efficacy and highlighting the need for glycosylation-aware immunogen engineering.
This study reveals that human T cells across diverse subtypes and individuals share conserved metabolic features, including high energy charge, robust antioxidant capacity, and abundant amino acid pools, which collectively prime them to meet the rapid energy and regulatory demands of an immune response.
This study presents the first high-resolution cryo-EM structure and genomic analysis of the podovirus {Phi}Xacm4-11, elucidating its unique T7-like architecture and molecular mechanisms for infecting the plant pathogen *Xanthomonas citri* to advance phage therapy applications.
The study demonstrates that the synthetic Coenzyme Q10 analog 6-Br-Q0C10 inhibits cell proliferation across multiple mammalian cell lines, particularly in obesity-related and cancer cells, suggesting its potential therapeutic application for managing obesity and retarding tumor growth.
This study utilizes combinatorial biosynthesis and genome mining to characterize cytochalasan cytochrome P450 monooxygenases, revealing that while these enzymes exhibit intrinsic promiscuity toward structurally related analogues, their activity on non-native substrates is strictly governed by the stereochemistry of functional groups rather than macrocycle size.
This computational study reveals that cancer-causing mutations in SHP-1 and SHP-2 disrupt the allosteric regulation of the catalytic WPD-loop dynamics by the SH2 domains, thereby altering enzyme activity and offering new insights for selective drug targeting.
This study establishes two complementary affinity-based workflows to optimize the purification of clusterin from human plasma and characterize its interactome, revealing its associations with immune response, hemostasis, and HDL-related pathways.
This study presents the first cryo-EM structure of the complete human KEOPS complex bound to its tRNA substrate and demonstrates that Galloway-Mowat syndrome pathogenic variants retain partial t6A modification activity in vitro and in yeast, indicating that the disease arises from suboptimal rather than total loss of t6A pathway function.
This paper describes the development of mRNA-BLNP3, a novel bifunctional lipid nanoparticle that combines CD1d and mannose receptor targeting to achieve superior antigen-presenting cell delivery, reduced liver accumulation, and enhanced humoral and cellular immune responses compared to previous generations.
This study establishes a fully circular chemo-enzymatic-microbial cascade that converts polyethylene into poly(3-hydroxybutyrate) by combining Baeyer-Villiger oxidation, machine learning-optimized enzymatic hydrolysis, and bioconversion using a newly isolated bacterial strain.
This study demonstrates that combining proteasome inhibitors with an autophagy-targeting chimera (AUTAC) synergistically enhances lysosome-mediated degradation of the anti-apoptotic protein Mcl1 by exploiting the NRF1-driven adaptive autophagy response, thereby overcoming drug resistance and promoting cell death in multiple myeloma and lung cancer models.
This study elucidates the crystal structure of the Drosophila adaptor protein Egalitarian bound to the K10 mRNA localization signal, revealing a modular binding mechanism that is essential for asymmetric mRNA transport and validated by functional deficits in genome-edited flies with mutations in the identified RNA-recognition residues.
Using a transient protein-RNA crosslink and Cryo-EM, researchers determined the 3.0 Å structure of the antibiotic resistance enzyme Cfr bound to an L-shaped rRNA fragment, revealing the structural basis for its unique C8 methylation activity.