388 papers
The authors developed a "TMT HR mode" for the Orbitrap Astral mass spectrometer that utilizes a multi-pass ion path to achieve the high resolving power necessary for accurate 35-plex Tandem Mass Tag proteomics, enabling deep, high-throughput quantification with precision comparable to gold-standard Orbitrap MS3 methods.
This study experimentally demonstrates that mutational robustness in protein function remains largely unchanged across various non-standard genetic codes constructed in vitro, suggesting that the standard genetic code's error-minimizing properties are not uniquely superior within the tested range of mutational costs.
This correspondence challenges the findings of a recent study by reporting that Protein Film Electrochemistry reveals the A559W and A559W/V610H mutants of CO dehydrogenase II do not exhibit the claimed enhanced oxygen resistance compared to the wild-type enzyme.
This study elucidates the catalytic mechanism of the PLP-dependent enzyme MilM, demonstrating that its essential homodimeric structure facilitates substrate binding, Cγ-hydroxylation of L-arginine via a superoxide intermediate, and a unique alternating lid mechanism for controlled substrate entry and product release during mildiomycin biosynthesis.
This study identifies a conserved hydrophobic interface involving specific leucine residues on GPCRs and their transducers (G proteins and -arrestins) that serves as a universal mechanism governing direct competition and receptor desensitization.
By integrating experimental data with computational modeling, this study elucidates the regulatory mechanisms of substrate transport, TCA cycle kinetics, and oxidative phosphorylation in cardiac mitochondria, revealing how ROS-dependent uncoupling and oxaloacetate accumulation influence metabolic flux and providing a comprehensive framework for simulating mitochondrial energy metabolism.
This study reveals that the irreversible chemical maturation of Fetuin-A-mineral complexes into distinct states acts as a critical regulatory switch, where small, labile monomers directly mineralize collagen while larger, matured particles require cellular uptake and lysosomal processing to release minerals, thereby separating mineral transport from physiological bone formation.
This study elucidates the near-complete biosynthetic pathway to the homoharringtonine core scaffold by identifying seven intermediates and six novel enzymes, revealing a whole-plant coordination model where the core is synthesized in root tips and distributed for further elaboration throughout the plant.
This paper details a laboratory's successful transition from X-ray crystallography to cryo-electron microscopy for studying the ATAD2B protein, outlining practical strategies to overcome biochemical and technical challenges while providing a comprehensive workflow guide for structural biologists adopting this transformative technology.
This paper investigates how combining two antagonistic integral controllers at a shared variable creates distinct regulatory modes—delegated, isolated, and metastable control—enabling both homeostatic and rheostatic behaviors, with applications explaining photoperiodic weight changes in Siberian hamsters and the complex dynamics of blood glucose homeostasis.
This study elucidates the molecular mechanism by which cell surface-anchored cell-penetrating peptides enable direct, non-endosomal protein delivery into living cells by locally hyperpolarizing the membrane to induce transient, selective water pores that preserve membrane integrity.
This study identifies a unique N-terminal cysteine motif in the mucin-type protein DPY-6 that facilitates its dimerization and species-specific localization, suggesting DPY-6 acts as a scaffold molecule for the assembly of the nematode cuticular extracellular matrix.
This study presents a fully flexible, textile-integrated electrochemical system comprising a laser-written glucose biosensor and a polyimide-based potentiostat that demonstrates stable, linear performance for wearable glucose monitoring in artificial sweat under mechanical stress.
This study identifies FAF1 and FAF2 as potent activators of the human p97-UFD1-NPL4 unfoldase complex, elucidating their structural mechanism for enhancing substrate unfolding and demonstrating how this knowledge enables the rational design of de novo proteins to boost p97 activity.
This study uses all-atom molecular dynamics simulations to reveal that while tetraethylammonium blocks Kv2.1 channels by binding on-axis near the selectivity filter, the specific inhibitor RY785 binds off-axis to the channel walls, stabilizing a semi-open gate state that occludes ion flow and influences distant voltage sensors.
This study validates a high-throughput ELISA assay demonstrating that the drug lumacaftor selectively inhibits von Willebrand factor binding under inflammatory, oxidizing conditions, offering a promising strategy for developing anti-thrombotic therapeutics that minimize bleeding risks.
This study successfully reconstructs and experimentally validates full-length ancestral schizorhodopsins and heliorhodopsins using a novel structure-guided, indel-aware sequence reconstruction method that overcomes alignment ambiguities to produce stable, retinal-binding proteins, thereby enabling direct investigation of how extra-membrane architectures evolve alongside the conserved transmembrane core.
This study demonstrates that Xer recombination at the plasmid pSC101 psi site proceeds with a precise topological linking number change of +4, converting four negative supercoils into catenation nodes via an antiparallel alignment and Holliday junction intermediate to ensure stable plasmid inheritance.
This study breaks the activity-stability trade-off in industrial PET hydrolases by genetically encoding azatryptophans to perform site-selective isosteric CH-to-N substitutions that enhance catalytic activity while preserving thermal stability, supported by a new rapid fluorescence-based kinetic assay.
This study overcomes the cytotoxicity barriers of snake venom metalloproteinases (SVMPs) by developing a generic recombinant zymogen production protocol using a baculovirus/insect cell system, which enables the safe expression, auto-activation, and functional characterization of all three SVMP classes for advancing haematology research and snakebite therapeutic discovery.