354 papers
This paper presents a modular protein-DNA co-crystal system that decouples crystal growth from guest protein installation, enabling the high-throughput structural determination and functional application of diverse DNA-binding proteins through programmable DNA scaffolds.
PAVR is a physics-aware light-field imaging platform that utilizes in silico training to enable high-resolution, high-throughput, and sample-independent 3D volumetric imaging of dynamic cellular processes without the need for external ground-truth data.
This paper introduces a single-shot, scanless temporal focusing quantitative phase microscopy (TF-QPM) technique that achieves high-speed, label-free, three-dimensional volumetric imaging with sub-micron optical sectioning and histology-level resolution, enabling rapid characterization of fast dynamics and virtual staining in complex biological samples.
This study utilizes cryo-electron microscopy to reveal that kinetically enhanced ribosomes evolved via oRibo-PACE achieve elevated translation rates through specific 16S rRNA mutations that introduce localized structural destabilization and RNA-protein rearrangements, rather than altering conserved catalytic centers.
This study demonstrates that fusing CRISPR-Csm complexes with the RNA ligase RtcB enables programmable RNA excision and the creation of chimeric transcripts ("spligation") in living cells, offering a novel method for precise RNA manipulation independent of canonical splice sites.
This study demonstrates that acidity, rather than propionate ions alone, is the primary driver of propionic acid-induced inhibition in anaerobic digestion, causing severe methane production loss and significant microbial community restructuring that correlates strongly with process failure.
This paper presents a modular, automated in-incubator epifluorescence microscope that overcomes the limitations of conventional manual and existing automated systems by enabling scalable, high-frequency, and thermally stable longitudinal live cell imaging with flexible multi-format and multi-fluorescence capabilities.
This study demonstrates that the environmental bacterium *Thauera aminoaromatica* S2 can be repurposed for gut-localized removal of the uremic toxin p-cresol by elucidating its anaerobic degradation pathway and validating its efficacy within a hydrogel-encapsulated system that functions within typical intestinal transit times.
This study demonstrates that a cardiac pulse signal, distinct from electrocardiographic artifacts, systematically contaminates local field potential recordings across diverse deep brain stimulation targets and patient groups, with spectral content extending into clinically relevant frequencies that necessitate careful screening for accurate biomarker detection and closed-loop therapy.
This study demonstrates that a fully reversible, tartrazine-based optical clearing strategy enables non-invasive, high-resolution, cortex-wide optical coherence tomography angiography (OCTA) of mouse brain vasculature through intact scalp and skull, eliminating the need for surgical excision.
By combining 2D and 3D X-ray scattering imaging to analyze 78 human femoral necks, this study reveals that the superior cortical region exhibits inferior bone quality characterized by misaligned mineral-collagen structures and disordered mineral platelets, which likely contribute to its heightened susceptibility to fracture independent of donor age or sex.
By combining deep mutational scanning with protein language models, researchers identified and experimentally validated myoglobin variants with significantly enhanced peroxidase activity, demonstrating that strategic aromatic substitutions facilitate long-range electron-hole hopping to improve catalytic efficiency.
The paper introduces Dynamic Co-Modulation (DyCoM), a unified operator framework that decomposes dynamic connectivity estimators into fundamental signal processing operations to resolve methodological fragmentation, explain divergent biological findings through distinct operator choices, and provide a principled foundation for future neuroimaging analysis.
This paper introduces and validates transient magnetic resonance elastography (tMRE) as a novel non-invasive technique capable of quantifying local myocardial stiffness at specific phases of the cardiac cycle in both rat models and phantoms, overcoming the spatiotemporal resolution limitations of current cardiac biomechanical assessment methods.
This paper presents a rapid, automated Raman spectroscopy method that achieves single-bead resolution and accurate quantification of residual immunomagnetic beads in cell therapy manufacturing, offering a robust alternative to time-intensive manual microscopy.
This study demonstrates that mechanical imbalances between collision and push-off impulses, driven by hip torque and speed-dependent work requirements, govern the asymmetry and timing of the vertical ground reaction force profile in human walking, leading to the proposal of a new metric (vGRF-TTI) for clinically assessing gait efficiency and neuromotor control.
This manuscript, which originally proposed a modular nanostructure design for DX-tile DNA nano-stars to control the self-organization and viscoelastic properties of DNA hydrogels, has been formally withdrawn following an investigation by George Mason University's Office of Research Integrity and Assurance.
This paper has been withdrawn following a formal review by George Mason University's Office of Research Integrity and Assurance, and therefore contains no scientific findings to summarize.
The authors developed a magnetically activatable baculoviral system that spatially confines CRISPR-mediated *Pdl1* disruption to tumors, thereby coupling localized innate immune activation with sustained checkpoint blockade to enhance combination immunotherapy while minimizing off-target effects.
This paper introduces Lattice Sequence Vascularization (LSV), a multiscale computational framework that generates hierarchical, biomimetic vascular networks for 3D-printed biohybrid tissues by integrating biological constraints, synthetic properties, and manufacturing requirements to overcome perfusion limitations in large-scale constructs.