Intensity and Landmark Based Registration Under Stain Normalization: A Comparative Study on Differently Stained Histopathology Images
This study demonstrates that a robust landmark-based registration technique utilizing thin-plate spline interpolation outperforms intensity-based methods like Elastix and ANTs in aligning histopathology images with varying stains, particularly when combined with deep learning-based stain normalization, despite the ongoing challenge of accurate landmark annotation.