816 papers
The paper introduces CELeidoscope, a novel quad-fluorescent *C. elegans* strain engineered via high-throughput screening that enables the simultaneous spectral sorting and transcriptomic profiling of four distinct major cell types within a single genetic background, thereby overcoming the limitations of traditional multi-strain approaches.
This study demonstrates that influenza A virus membrane fusion is actively regulated by the balance between hemagglutinin-receptor binding and neuraminidase-mediated receptor cleavage, where specific receptor interactions promote productive fusion-peptide insertion to enhance fusion efficiency.
This study reveals that the malaria parasite protein PfNCR1 relies on a unique amphipathic helix-linker-helix (HLH) domain, whose specific physicochemical properties enable its localization to narrow membrane contact sites, a process essential for maintaining cholesterol homeostasis and validating it as a drug target.
A one-year reciprocal transplant experiment in Alaskan tundra reveals that short-term variations in moss-associated nitrogen fixation are driven primarily by host species identity and microenvironment rather than by changes in the bacterial community structure, suggesting that host physiology plays a more critical role than microbiome turnover in near-term predictions of nitrogen inputs under Arctic climate change.
This study characterizes the *Campylobacter jejuni* T6SS as a critical virulence factor that mediates contact-dependent antagonism against competing bacteria via two novel effectors (CJ488_0980 and CJ488_0982) and enhances interaction with, invasion of, and survival within human intestinal epithelial cells.
By leveraging 1,150 complete genomes to construct accurate metabolic models, this study reveals how genomic traits drive distinct ecological interactions within the gut microbiome and demonstrates that targeting specific functional groups and keystone species significantly improves the diagnosis and understanding of inflammatory bowel disease compared to traditional community-level analyses.
This study demonstrates that global change drivers, particularly elevated CO2 and warming, decouple plant growth from rhizosphere enzymatic investment, thereby altering the competitive balance between invasive and native plants by reconfiguring above-belowground coupling and microbial resource acquisition strategies.
This study identifies YfhS and YjlC as novel regulatory partners that form a functional complex with the NADH dehydrogenase Ndh in *Bacillus subtilis*, revealing a critical mechanism for moderating NADH levels and preventing lethality that offers potential targets for new antibiotics against resistant bacteria.
This study reveals that *Yersinia pseudotuberculosis* microcolonies exhibit spatially distinct transcriptional responses to macrophage-derived factors, where peripheral bacteria primarily activate nitrosative stress and prophage genes while the entire colony upregulates itaconate degradation, though only a subset of colonies encounters sufficient itaconate to significantly impact bacterial fitness.
This study demonstrates that using human serum as a blocking agent and antibody diluent is a robust, cost-effective strategy to overcome Staphylococcus aureus Protein A-driven nonspecific antibody staining, thereby significantly improving the accuracy of immunofluorescence microscopy in infection models.
This study introduces the Alpine Lotic Phage (ALP) collection, a diverse set of 28 novel tailed phages isolated from alpine stream biofilms that infect 14 bacterial species and exhibit unique genomic and functional characteristics, thereby providing a foundational resource for exploring phage ecology and evolution in natural environments.
This study employs integrated omics analysis to reveal that the toluene-degrading bacterium *Acinetobacter* sp. Tol 5 adapts to gas-phase, water-limited conditions by reorganizing its metabolism to promote amino acid and nucleic acid degradation, accumulate citrulline as a stress response, and utilize intracellular storage lipids for survival.
This study identifies a novel Antarctic chemolithotroph, *Candidatus Mariimomonas ferrooxydans*, as a key driver of dark, anoxic iron biomineralization, providing genomic and functional evidence that challenges phototroph-centric models of banded iron formation and offering a modern analogue for understanding Earth's early geochemical history and potential extraterrestrial iron deposits.
This study demonstrates that reusable, immobilized benzyldimethyldodecyl ammonium chloride (BDMDAC) particles effectively reduce microbial and resistome burdens in wastewater post-treatment through a contact-restricted mechanism that achieves high lethality without promoting resistance selection or horizontal gene transfer.
By developing an automated device for continuous fecal sampling and combining it with advanced sequencing, researchers revealed that the mouse gut microbiome exhibits synchronized, reproducible daily rhythms driven by carbohydrate utilization strategies that are temporarily disrupted by perturbations but subsequently recover.
This study utilizes label-free quantitative proteomics to reveal that *Klebsiella pneumoniae* mounts a rapid, multifaceted adaptive response to colistin stress through outer membrane remodelling, lipid A modification, and metabolic re-routing, thereby identifying potential new therapeutic targets against multidrug resistance.
This study reveals that in growing *Escherichia coli* populations, the production of the biofilm matrix component colanic acid is spatially initiated at topological defects where cell orientation mismatches generate mechanical pressure, thereby demonstrating how cell orientation patterning organizes mechanical cues to trigger biofilm formation.
This study demonstrates that engineered phages, designed through defense-guided recombination to evade the complete repertoire of anti-phage systems in highly resistant MRSA clinical isolates, effectively prevent the emergence of bacterial resistance and offer a blueprint for rational next-generation phage therapeutics.
This study demonstrates that the clinical *Campylobacter jejuni* Bf strain utilizes selective secretion of larger, compositionally altered extracellular vesicles as a survival strategy to adapt to combined thermal and oxidative stress, thereby linking environmental resilience to enhanced epithelial toxicity and virulence.
This study elucidates the complex enzymatic pathway used by the gut symbiont *Bacteroides thetaiotaomicron* to degrade mucin O-glycans and demonstrates that multiple exo-acting enzymes targeting these structures are essential for successful gut colonization.