🧬 Category

q-bio

4605 papers

🧬 biology

High-level Expression and Integrated Purification of Recombinant Human Type I+III Collagen in Pichia pastoris

This study establishes a robust, scalable, and cost-effective manufacturing platform for recombinant human type I+III collagen in *Pichia pastoris* by combining multi-copy integration, optimized high-density fermentation at 22°C, and an integrated purification process to achieve high yields (10.42 mg/mL) with ≥94.7% purity and ≥90% activity recovery in a 500 L pilot-scale bioreactor.

Lu Li, Yue Huang, Xin Fu, He Xiao, Yun Zhang, Huaiqing Sun, Chaowan Guo, Ying Lin2026-07-01
🧬 biology

SSR marker-based genetic diversity and population structure of large-leaf tea germplasm across four major tea-producing regions of Yunnan, China

This study utilized SSR markers to analyze 216 large-leaf tea accessions from four Yunnan regions, revealing abundant genetic diversity primarily within populations and weak regional differentiation driven by extensive gene flow and historical exchange, thereby providing a foundation for future conservation and breeding efforts.

Qihuan Ding, Wuxiang Fu, Ning Chi, Jianmei Su, Shanshan Wu, Zhanxia Ma2026-07-01
🧬 biology

GcQCR7 Mediates Pathogenicity via Maintaining Cell Wall Integrity and Enhancing Oxidative Stress Tolerance in Glomerella cingulata

This study demonstrates that the mitochondrial subunit GcQCR7 is essential for *Glomerella cingulata* pathogenicity by maintaining cell wall integrity, energy metabolism, and oxidative stress tolerance, thereby identifying it as a novel target for managing Glomerella leaf spot in apple production.

Mingjuan Yang, Xinyue Cui, Shan Zhang, Fang Wang, Weichao Ren, Pingliang Li, Na Liu, Baohua Li, Chengying Jiang, Sen Lia (…)2026-07-01
🧬 biology

Mechanosensing at the endoplasmic reticulum by IRE1

This study identifies the endoplasmic reticulum as an autonomous mechanosensitive organelle where the transmembrane protein IRE1 directly senses membrane tension to trigger JNK signaling and enhance global protein synthesis, thereby linking mechanical forces to muscle adaptation independently of its canonical unfolded protein response role.

Hesso Farhan, Michaela Mayr, Luiz Garcia-Souza, Murphy McDermott, Utku Horzum, Yannick Frey, Margot Haun, Stephan Geley (…)2026-07-01
🧬 biology

Shotgun-NF: A reproducible Nextflow pipeline for end-to-end shotgun metagenomics analysis

Shotgun-NF is a portable, modular, and reproducible Nextflow pipeline that unifies a comprehensive suite of tools for end-to-end shotgun metagenomics analysis, from raw sequencing data to strain-level comparative insights, while ensuring execution consistency across diverse computing environments through containerization and automated provenance tracking.

Fereshteh Izadi, Sepideh Mofidifar, Emily A. Wasson, Carla S. Möller-Levet, André P. Gerber2026-07-01
🧬 biology

Predicting Microbiologically Influenced Corrosion Risk from Quorum Sensing Biofilm Community Features: A Random Forest-SHAP Approach

This study introduces a Random Forest-SHAP machine learning framework that successfully predicts microbiologically influenced corrosion risk by integrating novel quorum sensing biofilm community features with traditional environmental and microbial parameters, achieving an F1 score of 0.762 and demonstrating the predictive value of bacterial communication signals in corrosion assessment.

Bipul Bhattarai, Naina Maharajan, Sulav Dahal2026-07-01
🧬 biology

AI-Based Pipeline for Vascularization Detection in Histological Images; low-cost solution Using Moderate Computing Resources

This paper presents a low-cost, user-friendly AI pipeline that combines simple image processing with an interactive, self-learning similarity-search method to enable pathologists to rapidly and accurately detect vascularization in histological images using modest computing resources and minimal manual labeling.

Jan Žídek, Bretislav Lipový, Veronika Pavliňáková, Martin Knoz, Jakub Holoubek, David Izsák, Vladimir Váňa, Jan Herudek (…)2026-07-01
🧬 biology

Ecological Inference Is Structured Empirical Risk Minimization: Generalization Across Space, Nested Units, and Niche Support

This paper argues that the frequent failure of geographic models to generalize across sites stems from a fundamental estimand mismatch in nested survey data, demonstrating that ecological inference is structurally equivalent to empirical risk minimization under domain generalization and requiring population-level holdout validation rather than standard individual-level cross-validation to ensure reliable spatial transfer.

R. Craig Stillwell2026-07-01