346 papers
This paper presents a novel deep-tissue absolute force spectroscopy method that achieves sub-piconewton precision in optically turbid biological media by combining optical memory effect-based interferometry with a global fluctuation-dissipation fitting framework, enabling robust, quantitative in vivo measurements of forces and mechanical properties in living Drosophila tissues.
The paper introduces FOLIC, a novel foveated light-field compound imager that unifies wide-field context and single-cell resolution within a single capture to overcome traditional trade-offs in spatial resolution, field of view, and depth perception for biomedical applications.
By integrating a single-cell engineering workflow with molecular dynamics simulations, this study elucidates the structural dynamics mechanism—specifically enhanced receptor affinity and regulated heparan sulfate binding—that underpins the broad potency of the engineered AAV vector ATX002, thereby establishing a new framework for rational gene therapy vector design.
This systematic review and meta-analysis of 28 in vivo animal studies demonstrates that experimental intervertebral disc injury consistently leads to mechanical and morphological deterioration, identifying Young's modulus as a sensitive indicator of functional degeneration while highlighting the need for methodological standardization to improve clinical translation.
The study establishes a physiologically relevant, immunocompetent 3D organotypic model of the human respiratory mucosa that successfully supports influenza A virus replication and enables the monitoring of early antiviral innate immune responses.
The authors present OpenMebius2, a GUI-based software platform for 13C-metabolic flux analysis that utilizes low-cost tracer data to suggest optimal labeling patterns, thereby enhancing the precision of flux predictions.
The paper introduces Apollo-IRE1, a genetically encoded, ratiometric fluorescent sensor that enables live-cell, multiplexed imaging of ER stress dynamics by detecting IRE1 oligomerization through fluorescence anisotropy, offering a robust tool for studying beta-cell dysfunction in diabetes.
This study presents a chemically uncrosslinked, collagen-based bilayered tubular scaffold that mimics native tissue architecture to support vascular and airway regeneration, featuring a porous outer layer for cell colonization and a smooth inner layer for mechanical integrity and endothelialization, though its suture retention strength remains a limitation for in vivo use.
This study demonstrates that a one-dimensional convolutional neural network (1D-CNN) can accurately and generally predict cysteine concentrations across diverse pea cultivars using surface-enhanced Raman spectroscopy (SERS) spectra, offering a rapid alternative to traditional high-performance liquid chromatography (HPLC) methods.
This study presents a mechano-chemo-transductive strategy that reconciles the trade-off between exosome biogenesis and cellular internalization by engineering mesenchymal stem cells on soft matrices with ABCA1 modulation, resulting in highly potent exosomes that effectively treat pulmonary fibrosis through enhanced lung retention, macrophage repolarization, and fibrotic remodeling reversal.
This paper introduces plenoptic illuminated scanning laser ophthalmoscopy (PI-SLO), a high-speed, widefield 3D imaging modality that overcomes ocular aberrations to enable non-invasive, single-cell resolution volumetric imaging of retinal physiology in living eyes.
This paper introduces Gradient Embedded Multinozzle (GEM) printheads that combine parallelized printing with combinatorial ink mixing to accelerate the exploration and optimization of diverse ink formulations for complex 3D structures, as demonstrated through successful printing of cell-laden scaffolds and tunable hydrogel valves.
The authors developed VIVOS, a perfused 3D human vascular platform that reveals how physiological intraluminal flow drives endothelial state transitions and vessel remodeling through a YAP/TAZ-Apelin signaling switch, while also enabling the modeling of flow-regulated pathologies like arteriovenous malformations.
This study demonstrates that mechanical flexibility, decoupled from size and surface chemistry using DNA origami, serves as a critical design parameter enabling nanocarriers to overcome steric confinement and enhance transport through mucus barriers.
This study demonstrates that Raybloc, a marine bioactive silica-microsponge formulation, provides superior protection against blue light and infrared-A induced skin damage in mice by significantly reducing oxidative stress and inflammation while enhancing skin hydration and collagen preservation compared to hyaluronic acid-based products.
This study demonstrates that elastin-like polypeptide coacervate droplets can effectively sequester and remove heterogeneous incidental nanoplastics from solution by exploiting their dominant hydrophobic surface characteristics, achieving high capture efficiency across multiple cycles.
This study presents a programmable domestication strategy using the DNMB computational framework to identify and delete non-canonical defense systems in wild *Geobacillus* strains, thereby converting them into genetically tractable thermophilic hosts with a comprehensive engineering toolkit for industrial biotechnology.
This study demonstrates that elastin-like recombinamer (ELR)-gelatin hydrogels with imprinted micro- and nano-gratings significantly accelerate endothelial monolayer formation by synergizing bioactive matrix chemistry with surface topography to enhance early cell capture and promote rapid, stable alignment.
This study enhances the efficacy and safety of genetically delivered TGF-β monomers for cancer immunotherapy and fibrosis treatment by incorporating a modified pro-domain to correct folding issues and fusing a CD44-binding domain to achieve over 30-fold increased inhibitory activity on target cells.
This study demonstrates that engineered *Arabidopsis thaliana* plants can serve as noninvasive "sentinels" by converting belowground microbial activity into detectable aboveground signals through an optimized synthetic communication circuit responsive to p-coumaroyl-homoserine lactone.