351 papers
This study demonstrates that elastin-like polypeptide coacervate droplets can effectively sequester and remove heterogeneous incidental nanoplastics from solution by exploiting their dominant hydrophobic surface characteristics, achieving high capture efficiency across multiple cycles.
This study presents a programmable domestication strategy using the DNMB computational framework to identify and delete non-canonical defense systems in wild *Geobacillus* strains, thereby converting them into genetically tractable thermophilic hosts with a comprehensive engineering toolkit for industrial biotechnology.
This study demonstrates that elastin-like recombinamer (ELR)-gelatin hydrogels with imprinted micro- and nano-gratings significantly accelerate endothelial monolayer formation by synergizing bioactive matrix chemistry with surface topography to enhance early cell capture and promote rapid, stable alignment.
This study enhances the efficacy and safety of genetically delivered TGF-β monomers for cancer immunotherapy and fibrosis treatment by incorporating a modified pro-domain to correct folding issues and fusing a CD44-binding domain to achieve over 30-fold increased inhibitory activity on target cells.
This study demonstrates that engineered *Arabidopsis thaliana* plants can serve as noninvasive "sentinels" by converting belowground microbial activity into detectable aboveground signals through an optimized synthetic communication circuit responsive to p-coumaroyl-homoserine lactone.
This paper presents a streamlined, single-pot method for isolating target single-stranded DNA scaffolds from diverse double-stranded DNA sources using blocking oligonucleotides, enabling the direct and efficient assembly and testing of DNA origami structures without costly or time-consuming purification steps.
This study demonstrates that Ti3C2Tx MXene microelectrodes outperform conventional platinum electrodes in both recording and stimulation across various sizes (25–500 μm) due to reduced charge-transfer resistance and increased double-layer capacitance, with performance further enhanced by optimizing film thickness and concentration.
This paper presents a multi-objective optimization framework using particle swarm optimization to design a compliant, 3D-printed shoulder orthosis that effectively reduces muscle activity in healthy individuals while balancing gravity compensation with wearability constraints.
This study introduces and validates the optimized Multichannel Complexity Index (opMCI) as a novel metric for quantifying physiological synchrony, demonstrating its application to heart rate variability data reveals that a shared reading intervention significantly enhances parent-infant synchrony in preterm babies compared to play-based activities or full-term controls.
This paper presents a modified self-amplifying RNA (saRNA) platform that overcomes the limitations of traditional viral vectors and transient mRNA by enabling cost-effective, safe, and prolonged CAR T cell expression with enhanced tumor control and the capability for complex multi-genic logic functions.
This study demonstrates a scalable and modular platform for producing functional amyloid-based materials by engineering *Bacillus subtilis* spores to display TasA and squid suckerins on their surface, thereby overcoming limitations of traditional amyloid production while enabling applications such as enhanced 3D-printed resins.
This study identifies a conserved hyaluronan-centered extracellular matrix regulatory axis linking traumatic brain injury, spinal cord injury, and neural implant responses, suggesting that HA-associated signatures can serve as minimally invasive biomarkers for monitoring neural injury and implant biocompatibility.
This study systematically evaluated 48 intraperitoneally administered mRNA-LNP formulations in a pancreatic cancer mouse model to identify specific lipid compositions that decouple tumor accumulation from liver biodistribution, thereby establishing structure-activity relationships to guide the rational design of targeted mRNA therapeutics.
This paper evaluates and compares fully automated (nnU-Net) and prompt-based (MedSAM) segmentation models for trachea extraction from CT scans, demonstrating that dataset structure critically influences performance and proposing a hybrid strategy that leverages nnU-Net predictions to automatically generate prompts for MedSAM to enhance robustness and interpretability.
This paper presents a low-cost, rapid nanofluidic digital chip (Nano-dChip) utilizing duplex RT-LAMP to achieve highly sensitive, quantitative, and simultaneous detection of SARS-CoV-2 and Influenza H3 RNA within 30 minutes for effective point-of-care viral outbreak control.
This study demonstrates that SLIDE microscopy enables robust, high-speed 4D two-photon imaging of the enteric nervous system within intact, spontaneously contracting mouse intestines, overcoming the limitations of tissue opacity and physiological motion to visualize neural structures in their native 3D context without compromising tissue viability.
This study demonstrates that transglutaminase-mediated crosslinking of *Lupinus angustifolius* protein creates dose-dependent, elastic networks with reduced digestibility by targeting disordered structural domains, offering a rational multiscale approach to optimize plant proteins for sustainable food applications.
This study presents a novel 3D-printed hydrogel scaffold system that utilizes gelatin-based electrostatic interactions to achieve controlled, sustained release of BMP-9 from calcium sulfate microparticles while simultaneously encapsulating viable preosteoblasts, thereby creating an effective platform for enhanced bone regeneration.
This study demonstrates that bioimpedance monitoring provides a real-time, waveform-agnostic metric for tracking cardiac electroporation during pulsed field ablation, while revealing that the procedure-induced increase in tissue conductivity homogenizes myocardial anisotropy, thereby supporting simplified modeling and the derivation of validated lethal electric field thresholds.
This study demonstrates that a retaining GH1 -glycosidase can be engineered through targeted mutations of second-shell residues to acquire -glycosidase activity while maintaining its native -function, thereby proving that the stereochemical constraints of this enzyme family are more flexible than previously thought.