351 papers
This paper introduces a novel method for generating ventral and dorsal forebrain organoids that accurately recapitulate the macroscale geometry and tissue architecture of the developing telencephalon by utilizing an endothelial cell growth medium to expand the neuroepithelium and embedding the organoids in miniature collagen spheres to stabilize their structure and support neurovascular development.
This paper presents a scalable neural interface platform featuring a custom-designed 5,376-channel ASIC with integrated amplification, multiplexed ADCs, and stimulation capabilities, which successfully enables high-resolution, large-scale in vivo mapping of neural activity in rat brains through a gold bump-bonded flexible ECoG array.
This paper presents a semi-open microfluidic platform with 17,305 aligned wells that enables multi-marker profiling of extracellular vesicles released from single-cell-derived clones, revealing significant heterogeneity in EV signatures and their correlation with cellular proliferation.
This study demonstrates that Nutrishield's starch-based microencapsulation system significantly enhances the bioavailability and efficacy of beta-carotene, effectively attenuating LPS-induced systemic inflammation and oxidative stress in a murine model compared to non-encapsulated beta-carotene.
This study compares 3D vascular reconstructions from light sheet fluorescence microscopy, 4D ultrasound, and nano-computed tomography in chick embryos, revealing that while light sheet microscopy accurately captures quantitative volumetric measurements for computational modeling, 4D ultrasound systematically overestimates small tortuous vessels, leading to significant discrepancies in derived hemodynamic forces and flow patterns.
This study introduces a statistical BURST imaging framework that utilizes metrics like correlation and temporal contrast-to-noise ratio to suppress background noise and quantify detection confidence, thereby enabling high-fidelity, single-cell-level ultrasound imaging of biomolecules in complex in vivo environments.
This study demonstrates that a novel sublingual, orally disintegrating tablet vaccine (Capot) utilizing bacterial extracellular vesicles encapsulated in a calcium phosphate nanoshell effectively induces durable salivary sIgA immunity and provides robust protection against periodontitis in mice and non-human primates without causing inflammation.
This paper introduces ATPLyzer, a robust, genetically encoded, ratiometric multi-colour biosensor with a unique matryoshka design that enables minimally invasive, long-term, and single-wavelength excitation monitoring of intracellular ATP dynamics in living cells.
This study demonstrates that dynamic compressive loading of mesenchymal stromal cell spheroids within a dECM-reinforced alginate granular scaffold synergistically promotes hypertrophic cartilage formation via YAP1-mediated mechanotransduction, offering a cost-effective strategy for bone repair through endochondral ossification.
This study utilizes 3D hyaluronic acid-based hydrogels and inverse modeling to demonstrate that the self-assembly and contractile forces of human induced pluripotent endothelial progenitors are nonlinearly regulated by multicellularity, culture duration, and initial matrix stiffness, offering critical mechanical insights for engineering functional vascular networks.
This study demonstrates that 3D bioprinted GelMA-dECM liver scaffolds preserved at -80°C in an FBS-DMSO cocktail maintain approximately 80% cell viability and structural integrity, highlighting the potential of optimized cryopreservation protocols for creating ready-to-use liver models while underscoring the need for further refinement to mitigate preservation-induced injury.
This study demonstrates a carbon-negative, continuous mixotrophic gas fermentation process using *Cupriavidus necator* H16 to simultaneously assimilate CO2 and convert terephthalic acid into high-titre 2-pyrone-4,6-dicarboxylic acid, establishing a sustainable route for plastic monomer valorization despite challenges in producing pyridine dicarboxylic acids.
This study presents a genetically engineered biomimetic nanozyme (PB@ECM) that simultaneously scavenges reactive oxygen species and sequesters CXCL2 to reprogram inflammatory immune niches, thereby effectively inhibiting the transition from colitis to colorectal cancer in murine models.
This study utilizes simulations of SHG-informed fiber networks to demonstrate that while collagen fiber crimp does not influence diffusion tensor MRI metrics, fiber dispersion significantly alters axial and radial diffusivity as well as fractional anisotropy, thereby clarifying the relationship between microstructural variations and DTI measurements in tendons and ligaments.
This paper presents a computational model that simulates the interplay between geometry, mechanics, and stochasticity in tissue growth to enable the quantitative analysis of morphology and the inference of dynamic growth mechanisms from static experimental data, demonstrated through applications in 3D-printed scaffolds and cortical bone formation.
This preclinical study demonstrates that HipSAFE, a deep learning tool utilizing EfficientNet-Lite0 to analyze ultrasound images captured by non-expert operators, achieves diagnostic accuracy comparable to or exceeding that of radiologists for detecting hip fractures, offering a promising solution for improved triaging in rural and resource-constrained settings.
This study utilizes All Atom and Coarse Grained Molecular Dynamics simulations to elucidate and differentiate the endosomal escape mechanisms of various siRNA polyplexes and lipid nanoparticles, revealing the critical roles of hydrophobic residues and anionic lipids while successfully correlating these interaction patterns with in vitro performance.
This study utilizes multimodal surface-enhanced Raman spectroscopy and RNA sequencing of plasma extracellular vesicles to characterize molecular heterogeneity in type 2 diabetes, revealing distinct spectral and microRNA signatures across normal-weight and overweight subgroups of Asian and Non-Hispanic White patients.
Through evolutionary engineering of *Corynebacterium glutamicum*, researchers developed a high-performance strain with enhanced XylA and IolT1 activities that enables efficient whole-cell conversion of D-glucose to the low-calorie sweetener D-allulose at mesophilic temperatures, rivaling traditional high-temperature immobilized enzyme processes.
This study demonstrates that a conservative multi-echo ICA modeling approach, which orthogonalizes regressors to preserve task-related signals, significantly outperforms aggressive denoising methods in motor-task fMRI analyses, particularly when dealing with high task-correlated head motion and low signal-to-noise ratios.