816 papers
The study introduces LUNA, a novel coma-pattern-based autofocusing method that achieves nanoscale precision to overcome temperature-induced focus drift, thereby enabling the first single-cell resolution of bacterial cold shock response dynamics and revealing a continuous growth adaptation process that reconciles previously conflicting batch culture observations.
This study reveals that swine wastewater in Shandong Province, China, serves as a reservoir for diverse, multidrug-resistant carbapenemase-producing *E. coli* (including *bla*NDM, *bla*OXA-48-like, and *mcr*-1 co-harboring strains) with evidence of clonal spread across multiple cities, highlighting the critical need for continuous surveillance to protect human and environmental health.
This study demonstrates that an evolution-guided selection approach identifying a *Ruegeria* strain (MC10-B4) with genomic signatures of host dependency yields a persistent coral probiotic that enhances thermal tolerance through unique mechanisms like siderophore-mediated iron acquisition and a distinct motility-to-sessility reprogramming response, validating a strategy that prioritizes innate colonization potential over conventional laboratory traits.
This study presents a rapid, portable, and sensitive quantitative reverse-transcriptase recombinase polymerase amplification (qRT-RPA) assay targeting the sxtA4 transcript that enables early-warning detection of toxigenic Alexandrium species in under 15 minutes, overcoming the limitations of traditional microscopy and laboratory-based toxin analysis.
Two synthetic bacterial communities isolated from cereal rhizospheres effectively prime barley against powdery mildew by triggering defense responses without significantly disrupting native microbial community structure or function.
This study characterizes a collection of 133 Escherichia coli ST131 strains from Rio de Janeiro, revealing that subclade C2 exhibits high multidrug resistance and ESBL production while clades B and C2 possess distinct virulence profiles, collectively suggesting that Clade C strains are the primary drivers of ST131 dissemination and persistence in the region.
This study reveals that metabolic specialization shapes distinct gut bacterial niches in colorectal cancer, identifying the oral-associated bacterium *Leptotrichia wadei* as a pro-tumorigenic driver that promotes tumor growth by inducing M2 macrophage polarization.
This study demonstrates that the CLAMP-Linked Invasion Protein (CLIP) is essential for host cell invasion by both *Plasmodium berghei* merozoites and sporozoites, supporting a conserved function for the CLAMP-CLIP-SPATR complex across apicomplexan parasites.
This study demonstrates that interferon-beta acts as a central regulator of epithelial immune networks during *Chlamydia muridarum* infection, where its deficiency disrupts balanced inflammatory signaling and promotes fibrotic tissue remodeling in the female reproductive tract.
This study identifies a distinct immunoregulatory transcriptomic signature characterized by the upregulation of genes such as CCL22 and IDO1 in non-productively infected CD4+ T memory stem cells, suggesting this unique phenotype facilitates immune evasion and contributes to the persistence of the HIV reservoir.
This study reveals that the RstB-RstA two-component system specifically activates the OmrB sRNA and is subject to a novel dual feedback loop mediated by the asr-ydgU operon, where the small protein YdgU (renamed SamT) inhibits the system while Asr enhances its activity.
This study analyzes redox-stratified microbial mats from an early Proterozoic analog in Ethiopia to demonstrate that Asgard archaea thrive in sulfate-reduction zones through syntrophic interactions with sulfate-reducing bacteria, utilizing specific metabolic adaptations like oxygen-tolerant hydrogenases and methanogenesis while lacking aerobic respiration.
This study reveals that the ApeA defense system protects bacteria against single-stranded RNA phages by sensing viral RNA structures to activate its HEPN RNase domain, which directly cleaves the phage genome to halt replication without inducing cell death.
This study utilizes 104 novel viral genomic sequences to demonstrate that the H5N1 virus rapidly dispersed across 8,000 kilometers of the sub-Antarctic and Antarctica via long-distance movements of petrels and albatrosses, causing devastating impacts on marine wildlife while accumulating mammalian-adaptive mutations that raise concerns about its potential endemization and further evolution.
This study presents a novel alignment-free, k-mer guided pipeline that successfully identified conserved genomic targets to develop a highly sensitive and specific pan-Orthoflavivirus RT-qPCR assay, offering a scalable solution for global surveillance of diverse flaviviruses like dengue, Zika, and Japanese encephalitis virus.
This study demonstrates that live *Akkermansia muciniphila* VHAKM strain significantly enhances GLP-1 secretion in human L-cells more effectively than its pasteurized form or a commercial strain, highlighting a strain-specific and viability-dependent mechanism for improving metabolic health.
This study demonstrates that while pharmacological inhibition of METTL3 reduces m6A levels and attenuates HIV-1 latency reversal in CD4+ T cells, the three tested inhibitors (STM2457, STM3006, and STC-15) exhibit distinct profiles of potency and cytotoxicity, with STM2457 emerging as the least toxic option.
This study establishes that the transcriptional regulator DksA is a central integrator of the stringent stress response in *Klebsiella pneumoniae*, driving gastrointestinal colonization, virulence, and transmission by coordinating membrane stress resistance, capsule biosynthesis, biofilm formation, and RpoS regulation.
This multicenter retrospective study demonstrates that clinical shotgun metagenomic sequencing achieves high diagnostic accuracy for detecting fungi and parasites across various sample types, validating its performance against standard methods and establishing optimized, sample-specific read-based thresholds for standardized clinical implementation.
This study reveals that bacterial endosymbionts within the fungal pathogen *Fusarium oxysporum* enhance virulence by stimulating the production of beauvericin, which suppresses disease-inhibiting rhizosphere bacteria like *Streptomyces*, thereby disrupting the soil microbiome's natural disease-suppressive capabilities.