816 papers
This study establishes RfxCas13d as a potent and versatile programmable RNA-targeting antiviral platform that achieves broad-spectrum suppression of highly pathogenic avian influenza viruses, including H5N1, by targeting conserved viral RNA regions in avian cells.
This study demonstrates for the first time that azelaic acid functions as a dual antimicrobial and quorum sensing inhibitor against various pathogens, significantly attenuating virulence factors in *Pseudomonas aeruginosa* through dose-dependent mechanisms and molecular interactions with key regulatory proteins without directly inhibiting bacterial growth.
This paper introduces BertMS, a transformer-based framework that significantly improves spectral similarity estimation and molecular networking for the dereplication of unknown compounds in metabolomics by learning contextualized representations of fragment ions from large-scale MS/MS data.
This study reveals that in the bacterial symbiosis *Chlorochromatium aggregatum*, green sulfur epibionts synthesize and transfer unusually large virulence-factor-like proteins to their central partner, where they function to degrade protective capsules and form injection needles, thereby expanding the known evolutionary roles of bacterial virulence factors beyond pathogenesis.
This study demonstrates that the volatilomes of *Chromobacterium* species are most significantly influenced by whether they are cultured on solid versus liquid media, rather than by bacterial species or quorum sensing ability, highlighting the critical need to consider growth conditions in microbial volatilomics research.
This study identifies and characterizes a minimal consortium of six bacterial isolates from *Zostera marina* with complementary genomic and phenotypic traits for nitrogen, phosphorus, and sulfur cycling, as well as phytohormone production, offering a promising microbial strategy to enhance seagrass restoration efforts.
This study demonstrates that in *Pseudomonas aeruginosa* PAO1, inorganic polyphosphate metabolism critically regulates virulence by controlling pyoverdine synthesis, a mechanism effectively validated using zebrafish larvae as a robust and ethically aligned infection model.
This study demonstrates that the unfolded protein response regulator HAC1 is essential for *Candida auris* stress adaptation and virulence, as its unconventional splicing facilitates survival under ER and heat stress, while its deletion significantly reduces fungal burden and host mortality in infection models.
The study demonstrates that the extremotolerant rock-inhabiting fungus *Knufia petricola* modulates its growth, morphology, and biomass composition in response to carbon and nitrogen availability, switching to an explorative, filamentous growth strategy under nutrient limitation to reveal universal fungal resource-acquisition patterns.
This study utilized Illumina MiSeq metabarcoding to characterize fungal communities in Latvian faba beans, revealing that community diversity and composition are significantly influenced by sampling time (flowering vs. ripening) and the presence of *Alternaria*-induced leaf blotch, with *Alternaria* DNA detected even on asymptomatic leaves.
This study in food-secure Iringa, Tanzania, reveals that while age is the primary driver of gut microbiome diversity, stunted children exhibit distinct microbial enrichment patterns linked to poor hygiene and suboptimal dietary practices, suggesting that targeted improvements in feeding and WaSH interventions could mitigate growth failure despite adequate food availability.
This study demonstrates that while gene duplication-amplification in *Enterobacter cloacae* provides a dose-dependent survival advantage against ceftazidime, it imposes a physiological growth cost in the absence of antibiotics and paradoxically increases susceptibility to rapid killing at supra-MIC concentrations, highlighting the context-dependent trade-offs of this resistance mechanism.
This study demonstrates that *Rhodococcus* sp. ASF-10, a bacterium isolated from the Atlantic salmon gut, can biodegrade low molecular weight, oxidized polyethylene derivatives through specific enzymatic pathways, offering potential for sustainable bioremediation strategies.
This study demonstrates that the fluoroquinolone susceptibility of *Chlamydia trachomatis* is determined by stage-dependent DNA supercoiling levels, where moxifloxacin-induced disruption of coordinated gyrase and TopA activity drives developmental arrest and persistence phenotypes.
This paper presents a novel, efficient plasmid-based reverse genetics system for Murine Norovirus that utilizes vaccinia virus capping enzymes (D1R and D12L) and T7 RNA polymerase to significantly enhance viral rescue titers and polyprotein abundance in mammalian cells, offering a robust platform for high-throughput viral mutant analysis.
This study demonstrates that temperate siphonfish maintain a highly specific symbiosis with *Photobacterium mandapamensis* through host filtering, even as the bacterial symbionts exhibit significant strain-level genomic flexibility and phenotypic variation in bioluminescence.
This study characterizes the diverse microbiome of the deep Gourgouthakas Cave in Crete, revealing a rich reservoir of novel bacterial strains with significant potential for sustainable agriculture through the discovery of potent biocontrol agents and unique biosynthetic gene clusters.
This study elucidates the mechanism of the broad-spectrum bacteriocin RumC1, demonstrating that it targets a transient peptidoglycan intermediate in nascent cell walls to induce bacterial death, a process counteracted by the immunity protein RumIc1 which trims the critical stem peptide.
This study reveals that the translocation of gut bacteriophages into human circulation is a rare event governed by sequential barriers including epithelial passage, lymphatic trafficking, and antibody-mediated clearance, which collectively limit systemic phage persistence and shape the blood virome.
This study demonstrates that combining the IAP antagonist AZD5582 and BET inhibitor JQ1 to reverse HIV-1 latency, alongside Nef ablation to prevent immune evasion, effectively facilitates the immune-mediated elimination of latently infected T-cells, offering a promising strategy for an HIV cure.