816 papers
This study of Finnish wastewater *Escherichia coli* isolates reveals that while multidrug resistance and virulence factors do not correlate with biofilm formation, the presence of two or more heavy metal resistance genes is significantly associated with strong biofilm production.
This study identifies skeletocutin E, a fungal metabolite, as a specific dual-stage inhibitor of the apicoplast-localized *Plasmodium falciparum* pyruvate kinase II (PfPyrKII) that effectively blocks parasite growth in both liver and blood stages without affecting human pyruvate kinases, thereby validating PfPyrKII as a promising target for novel antimalarial therapies.
The paper introduces MATRIX, a high-throughput workflow combining automated microscopy, fluorescence staining, and Bayesian inference to rapidly and reproducibly quantify both total and active microbial cells while capturing single-cell phenotypes in diverse environmental microbiomes.
This study demonstrates that sediment-dwelling infauna selectively enhance the abundance, diversity, and transcriptional activity of DNA viruses through bacterial-mediated mechanisms, thereby establishing infauna as a critical regulator of viral dynamics and biogeochemical cycling in marine benthic ecosystems.
This study demonstrates that the Andes virus Gc glycoprotein requires a precise transmembrane domain length of at least 21 residues and a conserved polar serine residue (S1121) to successfully transition from membrane hemifusion to full fusion pore formation.
This study identifies the temperate bacteriophage {Phi}ESI as a key competitive factor in the emergent, multidrug-resistant *Salmonella* Infantis lineage, demonstrating that its prophage-mediated lysis of susceptible strains facilitates the lineage's ecological dominance and global spread.
This study demonstrates that the gut bacterium *Bacillus velezensis* T23 promotes zebrafish growth by releasing muropeptides that activate the NOD2 receptor, thereby triggering the IGF1-AKT/mTOR signaling pathway and enhancing intestinal cell renewal to drive systemic growth.
This study validates shoe sole dust as a reliable microbial sampler, revealing that bacterial and fungal communities respond to vegetation at different spatial scales, with bacteria linked to immediate path greenness and fungi to broader landscape-scale greenness.
This study reports the development of the first activity-based probes for detecting active IgA1 proteases in *Haemophilus influenzae* and utilizes them to identify and optimize a potent inhibitor that effectively blocks this key virulence factor's immune-evasion mechanism.
The study demonstrates that the lacritin-derived proteoform N-104 exerts a distinctive bactericidal effect on multidrug-resistant *P. aeruginosa* by binding to the lipoprotein YaiW to inhibit iron and polyamine transport, thereby triggering a broad transcriptional shift that suppresses virulence, fitness, and metabolic pathways while minimally overlapping with the mechanisms of conventional antibiotic classes.
By utilizing a high-density peptide array to analyze antibody responses, researchers comprehensively identified 283 immunogenic *Plasmodium vivax* proteins, including novel invasion candidates and unique asymptomatic response patterns, to advance vaccine development and serological diagnostics for malaria.
This study reveals that phages overcome the rapid diversification of *Pseudomonas aeruginosa* type IV pili through the modular structural diversification of their tail fibers, where distinct architectural strategies—ranging from conserved binding domains to highly variable regions—enable either specific or broad recognition of diverse pilin receptors.
The authors developed two tools, the cassette gatherer and hunter, which enable the high-throughput, sequence-independent recovery of thousands of uncharacterized integron cassettes from bacterial genomes, successfully facilitating the discovery of novel phage-defense systems.
This study demonstrates that the cyanobacterial enzyme BrtB captures free fatty acids directly at the cell envelope to form bartoloside fatty acid esters without prior activation, revealing a specialized, rapid-response pathway for exogenous fatty acid sequestration and metabolism.
Through an adaptive laboratory evolution study, researchers demonstrated that *Lactiplantibacillus plantarum* rapidly acquires and subsequently loses modest doxycycline resistance over 1000 generations, a process driven by specific, reversible mutations in the *rpsJ* gene encoding ribosomal protein S10.
This study demonstrates that microscale spatial fragmentation deterministically promotes bacterial survival under antibiotic exposure by creating physical refuges that slow bacterial growth and limit intracellular antibiotic accumulation, offering a non-genetic mechanism of protection applicable across various antibiotic classes.
This paper introduces the Small Volume Loader (SVL) for the SPOTs platform, a device featuring a pressure-compensating flared reservoir that enables precise, high-throughput droplet microarray loading with minimal dead volume, thereby allowing the screening of over 32,000 assays using 100-fold less scarce reagent than conventional methods.
This study demonstrates that the Fmr1 mutation in mice induces sex- and genotype-specific alterations in gut microbiome diversity and intestinal barrier integrity, characterized by reduced barrier gene expression in female knockout and heterozygous mice alongside increased epithelial resistance in heterozygous females.
This study identifies the FDA-approved antipsychotic Trifluoperazine as a potent broad-spectrum antiviral against arboviruses like JEV, DENV, and CHIKV that functions by inducing eIF2α phosphorylation and ER stress, thereby demonstrating significant therapeutic potential for repurposing in both in vitro and in vivo models.
This study reveals that the loss of ESX-1 genes in the smooth *Mycobacterium marinum* 1218S strain leads to increased transcription of ESX-6 and LOS genes, suggesting a regulatory link between these secretion systems, lipooligosaccharide synthesis, and colony morphology.