826 papers
This study demonstrates that Arp2/3 complex-dependent branched actin networks are essential for efficient RSV uncoating in A549 cells, as their disruption impairs viral genome uncoating and subsequent mRNA expression without affecting viral attachment or internalization.
This paper presents DropletFactory CORE, an affordable droplet cytometry and sorting platform designed to enable high-throughput, accessible single-cell screening of yeast libraries based on fluorescence signals for biotechnology applications.
Preliminary studies demonstrate that a beta-SARS-CoV-2 virus-like particle vaccine maintains structural integrity and immunogenicity at 4°C for 14 days and at -80°C for up to two years, with stability significantly enhanced by excipients such as polysorbate 80, sorbitol, or L-histidine compared to PBS alone.
This study introduces an interpretable computational framework utilizing differential geometry-based Directed Simplicial Neural Networks to integrate genomic and Cell Painting phenotypic data, successfully identifying specific genomic motifs and cellular morphology features as key drivers of antimicrobial resistance in *E. coli* and *K. pneumoniae* for potential rapid in silico diagnostics.
This study demonstrates that secondary bile acid metabolism, rather than nutrient competition, is the key mechanism by which specific Clostridia strains attenuate *C. difficile* toxin-mediated disease, suggesting that targeting virulence attenuation offers a promising strategy for next-generation therapeutics.
This metagenomic study of human gut microbiomes provides in vivo evidence that while ciprofloxacin induces prophages in specific bacterial species, it does not trigger a broad, microbiome-wide induction response.
This study identifies hypophosphite, a naturally occurring and safe inorganic compound, as a potent and selective inhibitor of syntrophic methanogenesis that targets formate metabolism, offering a promising strategy for controlling methane emissions in agricultural and natural environments without disrupting primary fermentation.
This study reveals that the gut bacterium *Clostridium asparagiforme* metabolizes methotrexate into DAMPA, a molecule that alleviates intestinal inflammation by activating mitochondrial STAT3 signaling to preserve epithelial integrity, thereby reframing microbial drug metabolism as an active regulator of host homeostasis.
This study demonstrates that *Photorhabdus* metabolites and associated soil legacy effects reshape soil microbial communities to enhance maize growth and induce strain-specific resistance against herbivorous insects.
*Toxoplasma gondii* infection activates the non-canonical NF-κB pathway by utilizing a collaborative network of eight MYR1-dependent effector proteins to deplete the host regulator TRAF3, thereby stabilizing NIK and processing p100 into p52.
This study identifies and characterizes RcrB, a previously unknown inner membrane protein in uropathogenic *Escherichia coli* that confers high-level resistance to host-derived reactive chlorine species by actively quenching extracellular hypochlorous acid through a glutathione-dependent mechanism.
This study identifies Sortase F as the essential bacterial enzyme in *Corynebacterium mastitidis* that anchors adhesins to the cell wall, enabling biofilm formation, ocular colonization, and the induction of protective IL-17-mediated immunity.
This study demonstrates that *Staphylococcus aureus* relies on orthogonal cell division to ensure the uniform surface distribution of specific adhesins, which is essential for organizing a protective fibrin capsule and enabling the formation of persistent staphylococcal abscess communities during infection.
Using transposon insertion sequencing on *Vibrio fischeri*, this study reveals that modeled microgravity has a minimal impact on bacterial gene essentiality, with only a few genes showing differential fitness requirements compared to normal gravity.
This study identifies a novel, rapid, calcium-dependent host cell entry pathway for *Staphylococcus aureus* that distinctively alters phagosomal maturation, promotes cytosolic translocation, and drives different bacterial replication and host cell death outcomes compared to slower internalization mechanisms.
By combining field monitoring with hydraulic modelling in Hyderabad's Musi River, this study reveals that untreated municipal sewage, rather than pharmaceutical manufacturing waste, is the primary driver of antimicrobial resistance, underscoring the urgent need for improved wastewater management in resource-limited urban environments.
This study reveals that the inner membrane protein SanA in *Escherichia coli* is essential for maintaining outer membrane integrity by modulating the balance of septal peptidoglycan synthesis, preventing envelope permeability defects that arise when increased lipid II availability disrupts cell division under stress conditions.
This study demonstrates that both genetic deletion and pharmacological inhibition of the peptidoglycan hydrolase SagA impair cell wall remodeling in vancomycin-resistant *Enterococcus faecium*, thereby restoring its susceptibility to vancomycin.
This pilot study demonstrates that household-scale urban Bokashi composting is dominated by beneficial lactic acid bacteria, effectively converts food waste into nutrient-rich organic acids for plant growth without significantly altering soil microbiomes or posing substantial health risks from pathogens.
This study reveals that the mycobacterial lipoglycan mannose-capped lipoarabinomannan (ManLAM) drives macrophage foam cell formation and lipid metabolic reprogramming in tuberculosis by simultaneously engaging Toll-like receptor 2 and Dectin-2 through distinct structural moieties, thereby activating an mTORC1-PPAR-dependent pathway that is largely independent of NF-B signaling.