829 papers
This study utilized ultradeep metatranscriptomic sequencing of 33 cats to demonstrate that feline oral inflammatory diseases are driven not by single pathogens but by community-wide metabolic remodeling involving shifts in species activity and the upregulation of stress, amino acid, and nitric oxide/polyamine metabolic pathways.
This study demonstrates that dual plasmepsin IX and X inhibitors possess a significantly higher barrier to resistance than plasmepsin X-selective inhibitors, as they could not be rendered ineffective through in vitro selection and retained efficacy against parasites that had developed resistance to the selective compounds.
This study demonstrates that early-life gut microbiomes from infants with later cognitive deficits causally induce metabolic and behavioral impairments in mice via reduced amino acid levels, which can be reversed by fecal transplants from healthy infants or a targeted microbial consortium.
This study demonstrates that manipulating the microbiota of male *Aedes aegypti* mosquitoes—specifically through axenic or monoxenic conditions—significantly enhances their longevity and mating efficiency in non-competitive settings, offering valuable insights for optimizing mass-rearing strategies for arbovirus control programs.
This study demonstrates that the CytoFLEX nano flow cytometer offers significantly improved scatter sensitivity compared to the conventional CytoFLEX S, enabling the detection of smaller viruses and previously undetectable extracellular vesicles within HIV preparations while maintaining comparable performance for surface protein labeling.
This study demonstrates that while Vancomycin-resistant *Enterococcus faecium* (VRE) inhibits *Clostridioides difficile* biofilm formation in the presence of fermentable glucose, stable dual-species biofilms can be established using non-fermentable sugars without altering vancomycin tolerance, and both single and dual-species biofilms can be dispersed through a nutrient step-change.
This study demonstrates that while both fall and spring prescribed burns in young mixed conifer forests promote the succession of fire-loving microbes, fall burns cause significantly greater and more prolonged reductions in soil microbial abundance and richness compared to spring burns.
This study reveals that the Epstein-Barr virus oncogene LMP1 confers resistance to ferroptosis in infected B cells by activating the TES2 signaling domain to upregulate PFKFB4, thereby enhancing pentose phosphate pathway flux and glutathione metabolism to bolster redox defense.
This large-scale metatranscriptomic study of 6,157 individuals demonstrates that the human gut microbiome exhibits remarkable temporal stability over periods ranging from hours to three years and rapidly recovers from major perturbations like colonoscopy preparation within one week, validating stool-based RNA sequencing as a robust tool for longitudinal health research.
This study establishes a long-term germ-free medaka model to demonstrate that while gut microbiota are essential for normal growth, immunity, and metabolism, sterile gut-derived metabolites can only partially rescue these deficits by enhancing specific metabolic pathways without fully restoring long-term survival or organ development.
This study reveals that the flagellar Type III secretion specificity switch is actively driven by the FliK-dependent removal of inhibitory components (Fluke and FlhBCCD) that maintain early secretion, rather than by passive sensing of assembly completion.
This study demonstrates that *Bacillus velezensis* GFZF-23 alleviates colitis by restoring microbiome homeostasis in conventional hosts and directly reprogramming host metabolism via -sitosterol in germ-free conditions, utilizing a reverse screening platform to disentangle these distinct therapeutic mechanisms.
This dual-center study demonstrates that integrating AI-based software (PhenoMATRIX) into fully automated microbiology laboratories significantly reduces urine culture turnaround times in both low-volume and high-volume settings, with additional gains achieved through automated result release.
This study identifies the mycobacterial ion channel Rv2571c as a critical driver of pyrazinamide resistance by mediating α-ketoglutarate efflux to counteract drug-induced cytoplasmic acidification, thereby establishing a novel mechanism for understanding and overcoming tuberculosis drug resistance.
The phage-encoded protein Aqs1 functions as a broad-spectrum inhibitor of bacterial type IV pili by binding to a conserved allosteric site on the PilB ATPase, which destabilizes its hexameric structure and prevents filament extension across diverse Gram-negative bacteria.
This study demonstrates that despite being expressed in vivo, the type II-secreted Exotoxin A (ToxA) is not essential for bacterial survival, neutrophil recruitment, or disease severity in murine *Pseudomonas aeruginosa* keratitis, contradicting earlier findings that suggested a critical role for this toxin.
This study presents a versatile and efficient genetic platform for non-model *Vibrio* species, utilizing RecA-mediated homologous recombination with dual counterselection systems (galK/DOG-2 and rpsL/streptomycin) and specialized vectors to enable targeted gene disruption, heterologous expression, and functional characterization of this ecologically and medically important bacterial genus.
The authors developed the High-Throughput Phage Isolation Platform (HtPIP), a scalable method using 0.2-micron filter plates that significantly reduces isolation time and cost while capturing a more diverse library of novel DNA and RNA phages across multiple bacterial genera compared to traditional low-throughput methods.
This multisite field study demonstrates that the real-world efficacy of antimicrobial coatings on public high-touch surfaces is highly context-dependent and cannot be reliably predicted by laboratory testing alone, with copper showing the most consistent performance while other materials like silver and SiQAC exhibited variable or negligible effects across different environments.
This study systematically identifies key genomic determinants, including receptor-binding proteins and anti-defence systems, that govern phage infectivity against *Pseudomonas aeruginosa* and leverages these features to train a machine-learning model that accurately predicts phage-host outcomes for the rational design of robust phage therapeutics.