838 papers
This study demonstrates that *Bacillus velezensis* GFZF-23 alleviates colitis by restoring microbiome homeostasis in conventional hosts and directly reprogramming host metabolism via -sitosterol in germ-free conditions, utilizing a reverse screening platform to disentangle these distinct therapeutic mechanisms.
This dual-center study demonstrates that integrating AI-based software (PhenoMATRIX) into fully automated microbiology laboratories significantly reduces urine culture turnaround times in both low-volume and high-volume settings, with additional gains achieved through automated result release.
This study identifies the mycobacterial ion channel Rv2571c as a critical driver of pyrazinamide resistance by mediating α-ketoglutarate efflux to counteract drug-induced cytoplasmic acidification, thereby establishing a novel mechanism for understanding and overcoming tuberculosis drug resistance.
The phage-encoded protein Aqs1 functions as a broad-spectrum inhibitor of bacterial type IV pili by binding to a conserved allosteric site on the PilB ATPase, which destabilizes its hexameric structure and prevents filament extension across diverse Gram-negative bacteria.
This study demonstrates that despite being expressed in vivo, the type II-secreted Exotoxin A (ToxA) is not essential for bacterial survival, neutrophil recruitment, or disease severity in murine *Pseudomonas aeruginosa* keratitis, contradicting earlier findings that suggested a critical role for this toxin.
This study presents a versatile and efficient genetic platform for non-model *Vibrio* species, utilizing RecA-mediated homologous recombination with dual counterselection systems (galK/DOG-2 and rpsL/streptomycin) and specialized vectors to enable targeted gene disruption, heterologous expression, and functional characterization of this ecologically and medically important bacterial genus.
The authors developed the High-Throughput Phage Isolation Platform (HtPIP), a scalable method using 0.2-micron filter plates that significantly reduces isolation time and cost while capturing a more diverse library of novel DNA and RNA phages across multiple bacterial genera compared to traditional low-throughput methods.
This multisite field study demonstrates that the real-world efficacy of antimicrobial coatings on public high-touch surfaces is highly context-dependent and cannot be reliably predicted by laboratory testing alone, with copper showing the most consistent performance while other materials like silver and SiQAC exhibited variable or negligible effects across different environments.
This study systematically identifies key genomic determinants, including receptor-binding proteins and anti-defence systems, that govern phage infectivity against *Pseudomonas aeruginosa* and leverages these features to train a machine-learning model that accurately predicts phage-host outcomes for the rational design of robust phage therapeutics.
This study revises the fungal class Archaeosporomycetes by proposing a new taxonomic structure that divides the previously single order Archaeosporales into four distinct orders—Archaeosporales, Ambisporales, Geosiphonales, and Polonosporales—based on robust phylogenetic support and significant morphological and ecological differences.
This study elucidates the mechanism of the DRT1 antiphage defence system, revealing that it synthesizes semirandom DNA adducts to trigger the assembly of dormant, filamentous structures that sequester a nitrilase effector, thereby activating an immune response against bacteriophages.
This study characterizes a 45 kDa chitinase from marine *Aspergillus terreus* that exhibits a unique dual mode of enzymatic action, functioning as both an endochitinase and an exochitinase to efficiently produce N-Acetyl-D-Glucosamine (GlcNAc) from chitin substrates.
This study combines solution NMR structural analysis with functional assays of vaccinia virus J5 mutants to identify a conserved P38YYCWY43 motif as essential for membrane fusion activity and a flexible 90-110 region as critical for the stable assembly of the viral entry-fusion complex.
This study reveals that the fungal regulator CtPHO4 gates virulence by repressing the colonization factor NFC1 during host phosphate starvation while inducing it under sufficiency, thereby coupling metabolic adaptation to infection strategies based on the host's internal nutrient status.
This study demonstrates that optimizing the ionic microenvironment with divalent cations and specific enrichment strategies overcomes adsorption barriers in Gram-positive bacteria, significantly enhancing the isolation efficiency and host range of therapeutic lytic phages against resistant *Staphylococcus* species.
This study employs integrated quantitative proteomics in both in-vitro and in-vivo models to reveal that *Candida auris* rapidly remodels its proteome—downregulating translation and rewiring metabolism, stress responses, and structural processes—to adapt to host immune pressure during early infection.
This study identifies the novel 2-benzimidazole derivative NR-5 as a promising, low-toxicity antimycobacterial candidate that exhibits potent activity against *M. smegmatis* and *B. subtilis* with favorable drug-likeness properties.
This study reveals that a proteostasis clock, driven by the dynamics of protein aggregate disassembly and the sequestration of the replication initiator DnaA, acts as a universal mechanism regulating bacterial lag time and antibiotic tolerance, thereby identifying proteostasis restoration as a critical target for combating dormancy.
This study demonstrates the feasibility of non-invasive fecal serology to detect prior Ebola virus exposure in free-ranging non-human primates in the Democratic Republic of Congo, providing the first evidence of cryptic viral circulation in *Cercopithecus ascanius* and *C. wolfi* despite the absence of active outbreaks.
This study reveals that extracellular vesicle-bound pneumolysin damages target cells through a noncanonical, pore-independent mechanism involving direct membrane fusion and destabilization, mediated by an exposed helix and cholesterol, rather than solely through traditional pore formation.