792 papers
This study develops difluoroaryl analogs of the gut bacterial L-dopa decarboxylase inhibitor AFMT that effectively block bacterial degradation of levodopa while minimizing unwanted metabolism by host tyrosine hydroxylase, thereby offering a promising strategy for improving Parkinson's disease treatment.
This study identifies the ENL-USP7 complex as a critical host factor that enforces HIV-1 latency by stabilizing BRD4 through deubiquitination, demonstrating that disrupting this axis with PROTACs effectively reactivates latent virus in diverse cellular models.
This study identifies five specific C-terminal residues (Ser83, Arg85, Tyr88, Asn124, and Lys192) of the *Mycoplasma synoviae* LAM hemagglutinin as critical for erythrocyte agglutination, demonstrating that their deletion compromises protein stability and function, particularly under acidic conditions.
This study introduces a novel, culture-independent metagenomic approach utilizing *Escherichia coli* minicells to enrich and sequence phages that successfully infect a target host, thereby effectively linking specific bacteriophages to their bacterial hosts and revealing a distinct, previously uncharacterized virome.
This study demonstrates that the heart failure drug levosimendan effectively inhibits HIV-1 infection and reactivation in various myeloid cells through a mechanism dependent on the serine/threonine kinase RIOK1, positioning it as a promising agent for block-and-lock strategies targeting myeloid viral reservoirs.
This study demonstrates that a triple-modality therapy combining bacteriophages, sub-minimal inhibitory concentration antibiotics, and antimicrobial peptides effectively eradicates multidrug-resistant *Serratia marcescens* biofilms by simultaneously targeting multiple cellular mechanisms, offering a promising solution for device-associated infections.
This study reveals that the CenIR system in *Clostridioides difficile* is an essential BlaIR-family regulator that maintains cell integrity by preventing lethal overproduction of the peptidoglycan hydrolase Cwp6, thereby demonstrating that BlaIR systems function broadly in environmental adaptation rather than solely in beta-lactam resistance.
The paper introduces V-SWITCH, a highly modular, single-vector, OFF-to-ON fluorescent reporter system that utilizes a viral protease-mediated release-and-capture mechanism to enable robust, real-time detection of diverse RNA virus infections at single-cell resolution for applications ranging from host factor screening to antiviral drug discovery.
This study reveals that in *Bacillus subtilis*, the replicative polymerase DnaE compensates for the absence of DNA polymerase I by utilizing strand displacement synthesis to efficiently repair Okazaki fragments, demonstrating the bacterium's ability to employ multiple mechanisms to maintain lagging strand replication fidelity.
The PmrB sensor in *Acinetobacter baumannii* detects sublethal oxidative stress via nickel oxidation to establish a response memory that primes antioxidant defenses and enhances virulence against subsequent lethal stresses and antimicrobial peptides.
This study reveals that heterocyst polysaccharide layer formation in *Anabaena* sp. PCC 7120 is controlled by a phosphorylation-dependent partner-switching mechanism where the kinase Alr3423 phosphorylates and inhibits the STAS domain protein All4160, while the phosphatase HenR dephosphorylates it to activate the biosynthetic pathway for nitrogen fixation.
This study demonstrates that the innate immune system, rather than the specific type or susceptibility profile of the antimicrobial agent, is the primary determinant of therapeutic success and survival in bacterial infections, challenging the traditional reliance on Minimum Inhibitory Concentration (MIC) for clinical treatment decisions.
This study demonstrates that high-light stress disrupts the local higher-order chromosome structure of the polyploid cyanobacterium *Synechocystis* sp. PCC 6803, as revealed by a newly established FISH method and corroborated by Hi-C analysis showing reduced short-range genomic interactions.
This study demonstrates that FK20, a random peptide mixture composed of L-phenylalanine and L-lysine, serves as a potent, broad-spectrum antifungal agent against multidrug-resistant pathogens like *Candida auris* by disrupting cell membranes, inhibiting biofilms, limiting resistance development, and showing therapeutic efficacy in a murine infection model.
This study demonstrates that human cytomegalovirus virions exhibit apparent cooperativity during cell infection, a phenomenon that invalidates conventional linear assumptions for calculating multiplicity of infection and necessitates a new methodology for accurate viral quantification.
This study systematically characterizes the bioenergetic diversity of nine hydrogenotrophic methanogens by demonstrating that variations in their H2 thresholds and growth yields correlate with energy conservation strategies, effectively distinguishing between cytochrome-containing and cytochrome-lacking groups while revealing further metabolic heterogeneity within these categories.
This study demonstrates that in the human fungal pathogen *Aspergillus fumigatus*, transient whole-chromosome aneuploidies serve as a flexible adaptive strategy to alleviate stress-induced growth defects and confer resistance to antifungal drugs by driving metabolic rewiring and the induction of silent biosynthetic gene clusters.
This study characterizes the versatile carbon metabolism of the industrial chassis *Acinetobacter* sp. Tol 5 by reconstructing its metabolic pathways and analyzing transcriptomic responses to diverse hydrocarbons and aromatics, revealing complex regulatory mechanisms such as the dual induction of catechol cleavage pathways during phenol degradation and the trade-off between rapid detoxification and carbon assimilation efficiency.
This study analyzes 1,027 *Proteus mirabilis* genomes to reveal that antimicrobial resistance is lineage-specific and driven by mobile genetic elements, yet often exhibits discordant genotype-phenotype correlations due to complex regulatory and stacking mechanisms, underscoring the need for genome-informed surveillance.
This study reveals that wastewater treatment plant discharges, particularly those containing hospital effluent, drive a progressive downstream increase in antimicrobial resistance genes along Switzerland's Aare River, while major tributaries sustain these elevated levels and lakes significantly reduce them.