3881 papers
This study demonstrates that a type IV collagen-enriched 3D extracellular matrix niche effectively preserves the mature phenotype and stable proliferation of primary mouse hepatocytes over extended culture periods while preventing the biliary metaplasia typically induced by conventional substrates like Matrigel.
This study developed and validated a highly sensitive, specific, and reproducible multiplex ddPCR assay capable of simultaneously quantifying tetracycline resistance genes (tetM and int-Tn) and differentiating between Ureaplasma urealyticum and Ureaplasma parvum to guide optimal clinical treatment strategies.
This study reconstructs the pig UGT gene family to reveal that while frozen ESM-2 embeddings improve the ranking of specific protein isoforms, they do not outperform traditional sequence-search methods in recovering additional genes once evaluation is standardized to the locus level, thereby exposing an isoform-resolution artifact in previous benchmarking.
This study demonstrates that engineering ribosome-binding site (RBS) strength in *E. coli* Nissle 1917 minicells allows for the precise balancing of recombinant protein expression levels and minicell yield, revealing that while higher expression reduces production due to metabolic burden, minicells provide a superior environment for maintaining protein solubility.
This study utilizes deep learning to classify over 800,000 CpGs into ten distinct DNA methylation aging patterns, revealing that genotype-specific methylation mediates inflammatory diseases while age-dependent epigenetic noise, driven by both immune-cell variations and cell-type-independent factors, is linked to inflammaging and mortality.
This study presents a high-confidence, curated genome annotation for the forest pathogen *Phytophthora plurivora* by integrating RNA-seq evidence and manual curation to eliminate automated artifacts, thereby recovering infection-induced effectors and confirming the species' two-speed genome architecture.
This study demonstrates that the naturally occurring flavonoid luteolin potently and reversibly inhibits human 5-HT3A receptor function through a non-competitive, voltage-independent mechanism involving specific residues I66 and W178, suggesting its potential as a scaffold for developing new receptor antagonists.
This study confirms the endemic presence of *Coxiella burnetii* in UAE dairy cattle through serological and molecular analysis, revealing active shedding in milk and genetically homogeneous strains that underscore the need for integrated One Health surveillance and control strategies.
This study advances wheat functional genomics by providing a complete telomere-to-telomere genome assembly and a large-scale indexed EMS mutant library of diploid einkorn wheat, which collectively overcome polyploid redundancy to enable rapid gene-to-trait associations.
This study employs TMT-based quantitative proteomics to identify 119 differentially expressed proteins between fertile and sterile *Echinococcus granulosus* cysts, revealing that enhanced protein synthesis and GPI-anchor biosynthesis pathways in fertile cysts likely drive their development and immune evasion, thereby offering potential targets for new anti-hydatid therapies.