4477 papers
This paper introduces deoxy-mononucleotide inhibited ligation (dMIL), a fully enzymatic and terminator-free single-nucleotide extension strategy for RNA oligonucleotides that utilizes engineered *E. coli* poly(A) polymerase (EcPAP) to exploit a unique 3′ terminal sugar specificity for precise, high-fidelity synthesis.
This study reveals that the widely used A50I vinculin mutant retains residual talin binding, prompting the development of a superior I12K/A50I mutant which demonstrates that while talin is not required for vinculin to experience mechanical load, it is essential for recruiting vinculin to focal adhesions and organizing the spatial distribution of tension within these complexes.
This study optimizes the plastination technique for avian specimens by demonstrating that room-temperature processing combined with post-treatment absorption and adsorption methods effectively preserves the morphological integrity and natural appearance of bird plumage, thereby overcoming a decades-long challenge in preserving this diverse vertebrate group.
Chronic intraperitoneal exposure to silica nanoparticles in rats induces significant neurobehavioral deficits, including anxiety and memory impairment, alongside widespread histological damage and neuronal degeneration across multiple brain regions, highlighting the material's potent neurotoxicity.
This paper presents a cell-centric alignment framework using Coherent Point Drift and graph matching to integrate H&E and multiplex immunofluorescence images in TMA cores, enabling spatial co-localization, feature validation, and the generation of virtual H&E for improved tumor microenvironment analysis.
This study utilizes RNA-seq and bioinformatic analysis to identify the upregulated FOS gene as a potential therapeutic target for hypertensive intracerebral hemorrhage, which may promote neural recovery by activating neural stem cells through the miR-21/SOX2 signaling pathway.
This study reveals that while five migratory passerine species share similar localized movement strategies during post-breeding stopover, they reduce competition by partitioning resources through differences in daily activity timing and fine-scale habitat preferences driven by forest structure.
This study demonstrates that interpretable machine learning models can successfully discriminate between Concentrative, Loving-Kindness, and Emptiness meditative phases across different subjects using EEG-derived temporal connectivity patterns, particularly delta-band coherence, thereby establishing a foundation for objective, subject-independent meditation feedback tools.
This study demonstrates that the in ovo administration of calcium carbonate nanoparticles accelerates femoral osteogenesis and promotes bone mineralization in chick embryos, as evidenced by advanced histological development, increased expression of osteogenic markers, and progressive radiographic mineralization.
This study demonstrates that high-fidelity BCI decoding during postural motor imagery relies on local sensorimotor rhythms from the dominant hand area rather than global brain network efficiency, revealing a task-signal mismatch where performance is decoupled from whole-brain integration and driven by a dynamic, M1-centric information-broadcasting network.